Product Name	FLII
Chinese Name	蛋白质flightless-1同源物抗体
Alias	Fli 1; FLI; Flightless-1; Flightless1; Flightless 1; Flightless I (Drosophila) homolog; Flightless I homolog; Flightless I homolog (Drosophila); Flightless1; FlightlessI; Fliih; MGC39265; Protein flightless 1 homolog; FLI1_HUMAN.
Research Area	Cell biology  immunology  Stem cells  Cyclin  Cell differentiation  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	51kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Flightless 1: 343-452/452
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Mutations in this gene leads to abnormal muscle function, arrested development and embryonic lethality. The protein sequence shows that this might be a regulator of cytoskeleton and may have a role during cell division. Function: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Subunit: Can form homodimers or heterodimers with ETV6/TEL1. Subcellular Location: Nucleus. DISEASE: Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Similarity: Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain. SWISS: Q13045 Gene ID: 2314 Database links: Entrez Gene: 2314 Human Entrez Gene: 14248 Mouse Entrez Gene: 287375 Rat Omim: 600362 Human SwissProt: Q13045 Human SwissProt: Q9JJ28 Mouse Unigene: 513984 Human Unigene: 339755 Mouse Unigene: 144698 Rat
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti- Flightless 1 (SL7864R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Flightless 1) Polyclonal Antibody, Unconjugated (SL7864R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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