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Product Name FAM3C Chinese Name 上皮分泌型FAM3C蛋白抗体 Alias Fam3c; FAM3C_HUMAN; family with sequence similarity 3, member C; GS3786; ILEI; Interleukin-like EMT inducer; Predicted osteoblast protein; Protein FAM3C. Research Area Tumour Cell biology immunology Cyclin Cell differentiation Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 22kDa Cellular localization cytoplasmic Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FAM3C: 151-227/227 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ILEI is a 227 amino acid, ubiquitously expressed protein containing an amino-terminal signal peptide. Elevated levels of ILEI translation are observed in oncogenic, Ras-transformed mammary epithelial cells and epithelial to mesenchymal transition (Emt) as well as tumor growth and metastasis. Also, overexpression of ILEI results in loss of ZO-1, a protein involved in tight junctions, and expression of cytoplasmic E-cadherin, which has been shown to influence loss of polarity and invasiveness. Due to this evidence, it is suspected that ILEI cooperates with oncogenic Ras to cause TGF∫-independent Emt and its overexpression is correlated with the invasion, metastasis and survival in a variety of cancers.
Function:
May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.
Subcellular Location:
Secreted. Cytoplasmic vesicle. Note=Cytoplasmic in some cancer cells.
Tissue Specificity:
Present in most secretory epithelia (at protein level).
Similarity:
Belongs to the FAM3 family.
SWISS:
Q92520
Gene ID:
10447
Database links:
UniProtKB/Swiss-Prot: Q92520.1
Product Picture Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti-FAM3C (SL7862R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAM3C) Polyclonal Antibody, Unconjugated (SL7862R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FAM3C) Polyclonal Antibody, Unconjugated (SL7862R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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