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Product Name Cytochrome b245 Light Chain Chinese Name 细胞色素b245轻链抗体 Alias CYBA; Cytochrome b 245 alpha polypeptide; Cytochrome b 245 light chain; Cytochrome b alpha polypeptide; Cytochrome b(558) alpha chain; Cytochrome b(558) alpha subunit; Cytochrome b558 subunit alpha; Flavocytochrome b 558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; CY24A_HUMAN; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit; p22-PHOX. literatures Specific References (6) | SL3879R has been referenced in 6 publications.[IF=5.81] Xiaoyu Liu. et al. Geniposide Combined With Notoginsenoside R1 Attenuates Inflammation and Apoptosis in Atherosclerosis via the AMPK/mTOR/Nrf2 Signaling Pathway. Front Pharmacol. 2021; 12: 687394 WB ; Mouse, Human.[IF=3.73] Walton, Noah M., et al. "Adult neurogenesis transiently generates oxidative stress." PloS one 7.4 (2012): e35264. IHC-F ; Mouse.[IF=3.457] Song Q et al. Potential mechanisms underlying the protective effects of salvianic acid A against atherosclerosis in vivo and vitro. (2019)Biomedicine & Pharmacotherapy, 109, 945–956. WB ; Rat.[IF=2.99] Li, A., et al. "Arctigenin suppresses transforming growth factor-β1-induced expression of monocyte chemoattractant protein-1 and the subsequent epithelial-mesenchymal transition through reactive oxygen species-dependent ERK/NF-κB signaling pathway in renal tubular epithelial cells." Free Radical Research (2015): 1-19. other ;[IF=2.86] Yue, Long, et al. "Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways." International Journal of Biological Macromolecules (2016). WB ; Human.[IF=1.72] Yoshida, Toshinori, et al. "Apocynin and enzymatically modified isoquercitrin suppress the expression of a NADPH oxidase subunit p22phox in steatosis-related preneoplastic liver foci of rats." Experimental and Toxicologic Pathology (2016). IHC-P ; Rat.Research Area Tumour Cell biology immunology lymphocyte t-lymphocyte The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 21kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CK18 Cytochrome b245 Light Chain/p22phox: 21-120/195 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions.
Function:
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
Subunit:
Composed of a heavy chain (beta) and a light chain (alpha). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9).
Subcellular Location:
Cell Membrane
Post-translational modifications:
The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245. [PTM] Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding (By similarity).
DISEASE:
Granulomatous disease, chronic, cytochrome-b-negative, autosomal recessive (ARCGD) [MIM:233690]: A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the p22phox family.
SWISS:
P13498
Gene ID:
1535
Database links:Entrez Gene: 1535 Human
Entrez Gene: 13057 Mouse
Omim: 608508 Human
SwissProt: P13498 Human
SwissProt: Q61462 Mouse
Unigene: 513803 Human
p22phox又称中性粒细胞色素b22kDa的多肽,主要用于Cardiovascular病及心肺功能的研究。Product Picture Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CYBA Polyclonal Antibody, Unconjugated(SL3879R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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