Product Name	AQP7
Chinese Name	水Channel protein-7抗体
Alias	aquaporin Protein-7; AQP7; AQP7L; AQP9; AQPap; Aqpap7; Aquaporin 7;Aquaporin-7 like; MGC149555; MGC149556; AQP7_HUMAN.
literatures	Specific References  (2)     |     SL2506R has been referenced in 2 publications. [IF=5.195] Mei-Mei Zhang. et al. Time-dependent laxative effect of sennoside A, the core functional component of rhubarb, is attributed to gut microbiota and aquaporins. J ETHNOPHARMACOL. 2023 Jul;311:116431  WB ;  Mouse.   PubMed:37003403 [IF=1.21] Vicente‐Carrillo, A., et al. "Membrane Stress During Thawing Elicits Redistribution of Aquaporin 7 But Not of Aquaporin 9 in Boar Spermatozoa."Reproduction in Domestic Animals (2016).  IF(ICC) ;  Other Species.   PubMed:27405395
Research Area	Cell biology  immunology  Channel protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	37kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human AQP7: 251-342/342 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin 1 (AQP1, purified from red cells) also called CHIP28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CATFR or Shriveled) is a transposon induced splicing error that substitutes a long terminal repeat sequence for the C terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane. Function: Forms a channel for water and glycerol (By similarity). Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. SWISS: O14520 Gene ID: 364 Database links: Entrez Gene: 364 Human Entrez Gene: 11832 Mouse Entrez Gene: 29171 Rat Omim: 602974 Human SwissProt: O14520 Human SwissProt: O54794 Mouse SwissProt: P56403 Rat Unigene: 455323 Human Unigene: 8728 Mouse Unigene: 11111 Rat
Product Picture	Sample: Lane 1: Adipose (Mouse) Lysate at 40 ug Lane 2: Kidney (Mouse) Lysate at 40 ug Lane 3: Testis (Mouse) Lysate at 40 ug Lane 4: Spleen (Mouse) Lysate at 40 ug Primary: Anti-AQP7 (SL2506R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37/18 kD Observed band size: 40 kD Tissue/cell:Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-AQP7 Polyclonal Antibody, Unconjugated(SL2506R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Black line : Positive blank control (A549); Negative blank control (HUVEC） Green line : Primary Antibody (Rabbit Anti-AQP7 antibody (SL2506R) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488) A549（Positive）and HUVEC（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AQP7 Antibody(SL2506R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). Blank control: A549. Primary Antibody (green line): Rabbit Anti-AQP7 antibody (SL2506R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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