Product Name	Dopamine Transporter
Chinese Name	多巴胺TransporterDAT抗体
Alias	DA transporter; DAT 1; DAT; DAT1; SC6A3_HUMAN; SLC6A3; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3; Solute carrier family 6 member 3; Variable number tandem repeat (VNTR); dopamine transporter; ADAT 1; Adenosine deaminase tRNA specific 1; HADAT1; tRNA specific adenosine deaminase 1.
literatures	Specific References  (10)     |     SL1714R has been referenced in 10 publications. [IF=11.799] Chenyu Zhang. et al. The Nrf2-NLRP3-caspase-1 axis mediates the neuroprotective effects of Celastrol in Parkinson's disease. Redox Biol. 2021 Nov;47:102134  WB ;  mouse.   PubMed:34600334 [IF=6.751] Maria Teresa Rivera-Garciaet al. Sex and strain differences in dynamic and static properties of the mesolimbic dopamine system. Neuropsychopharmacology . 2020 Nov;45(12):2079-2086.  IHC ;  mouse.   PubMed:32663840 [IF=6.543] Huang Jian. et al. Icariside II Attenuates Methamphetamine-Induced Neurotoxicity and Behavioral Impairments via Activating the Keap1-Nrf2 Pathway. Oxid Med Cell Longev. 2022;2022:8400876  WB ;  Mouse.   PubMed:35387263 [IF=4.868] Li R et al. Salidroside Protects Dopaminergic Neurons by Enhancing PINK1/Parkin-Mediated Mitophagy. Oxid Med Cell Longev. 2019 Sep 10;2019:9341018.  WB&ICF\IHF ;  Mouse.   PubMed:31583052 [IF=4.831] Shi L et al. Clioquinol improves motor and non-motor deficits in MPTP-induced monkey model of Parkinson's disease through AKT/mTOR pathway. Aging (Albany NY) . 2020 May 18;12(10):9515-9533.  WB ;  Monkey.   PubMed:32424108 [IF=4.181] Bai L et al. m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite.Toxicol Sci. 2018 Oct 1;165(2):431-446.  WB&ICF ;  Mouse&Rat.   PubMed:29982692 [IF=3.31] Ni, Na, et al. "Self-Assembling Peptide Nanofiber Scaffolds Enhance Dopaminergic Differentiation of Mouse Pluripotent Stem Cells in 3-Dimensional Culture." PLOS ONE 8.12 (2013): e84504.  IF(ICC) ;  Mouse.   PubMed:24376815 [IF=2.57] Romei, Cristina, et al. "Colocalization of neurotransmitter transporters on the plasma membrane of the same nerve terminal may reflect cotransmission."Brain Research Bulletin (2016).  IHC ;  Mouse.   PubMed:27565422 [IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25.  IHC-P ;  rat&mink.   PubMed:31768887 [IF=0.64] Qian, Weibin, et al. "Effect of Gingerol on Cisplatin-Induced Pica Analogous to Emesis Via Modulating Expressions of Dopamine 2 Receptor, Dopamine Transporter and Tyrosine Hydroxylase in the Vomiting Model of Rats."Yonago Acta medica 59 (2016): 100-110.  IHC-F ;  Rat.   PubMed:27493480
Research Area	Cell biology  immunology  Neurobiology  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, )
Applications	WB=1:500-2000（Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	68kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DAT1: 579-620/620 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009] Function: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Subunit: Homooligomer; disulfide-linked. Interacts with PRKCABP and TGFB1I1. Interacts (via N-terminus) with SYNGR3 (via N-terminus). Interacts with SLC18A2. Subcellular Location: Membrane; Multi-pass membrane protein. DISEASE: Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Similarity: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily. SWISS: Q01959 Gene ID: 6531 Database links: Entrez Gene: 6531 Human Entrez Gene: 13162 Mouse Entrez Gene: 24898 Rat Omim: 126455 Human SwissProt: Q01959 Human SwissProt: Q61327 Mouse SwissProt: P23977 Rat Unigene: 406 Human Unigene: 41993 Mouse Unigene: 10093 Rat DATI 可能参与神经系统的多种活动，如学习记忆、嗅觉、感觉、运动、多巴胺神经递质活动的调节，而且可能参与胶质瘤等神经系统Tumour的发生,阳性着色主要定位于细胞质，但也可见到有些The nucleus内的阳性染色.
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Dopamine Transporter (SL1714R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated (SL1714R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated (SL1714R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control: U937 (blue). Primary Antibody:Rabbit Anti-Dopamine Transporter antibody(SL1714R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (SL1714R, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. Positive control: RSC96 Isotype Control Antibody: rabbit IgG-PE/CY5, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μg in 100 μL1X PBS containing 0.5% BSA. Positive control: RSC96 Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody catalog number: SL1714R; Dilution: 12μg in 100 μl 1X PBS containing 0.5% BSA

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