Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

Mycoplasma canis is a commensal bacterium of the respiratory and urogenital tracts of dogs that can be isolated from the mucosal surfaces of dogs or cattle. Can cause opportunistic infections and can infect many mammals. Mycoplasma canis has been reported to be found in the brain tissue of dogs and may be associated with granulomatous and necrotizing meningoencephalitis. Occasionally there are reports that Mycoplasma canis can parasitize the respiratory tract of immunocompromised patients and that dog bites may cause human infection.


Mycoplasma canis can be identified by in vitro culture and serological methods, but culture The method is more time-consuming and has more cross-reactions with serological methods. The PCR method is used for identification, which has extremely high sensitivity and specificity, and the detection time is short, and the results can be obtained in just a few hours.


This kit selected the 16S rRNA gene for PCR identification, and the primers were verified by BLAST To specifically target Mycoplasma canis, only Mycoplasma bovirhinis may cause interference at high concentrations and has no cross-reactivity with other organism genomes. The host of Mycoplasma bovis is different from that of Mycoplasma canis, which generally does not affect the detection. Thirteen species of mycoplasma and 3 species of bacteria were detected. Only Mycoplasma canis produced specific bands, and no other strains were amplified. Thirteen positive results were detected from 59 canine tracheal tissue samples. It can be seen that this kit is specific for Mycoplasma canis and can be used for detection and identification.


This product is for scientific research only.

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

犬支原体（Mycoplasma canis）是犬的呼吸道和泌尿生殖道共生菌，可从犬或牛的粘膜表面分离出来。可引起机会型感染，可以感染很多哺乳动物。 有报道在犬的脑组织中可发现犬支原体，可能与肉芽肿性和坏死性脑膜脑炎有关。偶有报道显示，犬支原体可寄生于免疫缺陷病人的呼吸道，以及犬咬可能致人感染。

犬支原体可以用体外培养法和血清学方法进行鉴定，但培养法较为耗时，血清学方法交叉反应较多。使用PCR法进行鉴定，具有极高的灵敏度和特异性，且检测时间短，只需数小时即可获得结果。

本试剂盒选取了16S rRNA基因进行PCR鉴定，引物经BLAST验证为特异性靶向犬支原体，仅牛鼻支原体（Mycoplasma bovirhinis）在高浓度时可能造成干扰，与其他生物基因组无交叉反应。牛鼻支原体的宿主与犬支原体不同，一般不会对检测造成影响。检测13种支原体（不包括牛鼻支原体）和3种细菌，只有犬支原体产生特异性条带，其他菌株均没有扩增。从59个犬气管组织样品中检出13个阳性。可见本试剂盒对犬支原体有特异性，可用于检测和鉴定。

本产品仅供科学研究使用。

 

 

 

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