Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma edwardsiana is generally considered to be a mycoplasma that parasitizes dogs. It was originally isolated from the mouth of dogs and was later found in the lungs, trachea, oropharynx, tonsils, cervix, prostate, foreskin, Many parts such as the vagina, nasal cavity and lymph nodes can be isolated. In a Japanese study, it was also isolated from the oral cavity, nasal cavity, and rectum of wild healthy raccoons. Mycoplasma edwardsiana can be carried by both healthy and sick dogs and was initially thought to be associated with pneumonia. It has also been found in dogs with polyarthritis and sepsis.


Mycoplasma edwardsiana can be identified by in vitro culture and serological methods, but culture The method is more time-consuming and has more cross-reactions with serological methods. The PCR method is used for identification, which has extremely high sensitivity and specificity, and the detection time is short, and the results can be obtained in just a few hours.


This kit selected the 16S rRNA gene for PCR identification, and the primers were verified by BLAST It specifically targets Mycoplasma edwardsiana and has no cross-reactivity with other biological genomes. Thirteen species of mycoplasma and 3 species of bacteria were detected. Only Mycoplasma edwardsiana produced specific bands, and no other strains were amplified. 14 positive results were detected from 58 canine tracheal tissue samples. It can be seen that this kit is specific for Mycoplasma edwardsiana and can be used for detection and identification.


This product is for scientific research only.

 

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-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

爱德华支原体（Mycoplasma edwardii）一般认为是寄生于犬类的支原体，最初是从犬的口腔中分离出来，后来发现在犬的肺、气管、口咽部、扁桃体、子宫颈、前列腺、包皮、阴道、鼻腔和淋巴结等很多部位都可以分离得到。在日本的一项研究中，从野生健康浣熊的口腔、鼻腔和直肠中也可分离得到。健康和患病的犬均可携带爱德华支原体，早期认为其与肺炎有关，后来在患多发性关节炎和败血症的犬中也发现了爱德华支原体。

爱德华支原体可以用体外培养法和血清学方法进行鉴定，但培养法较为耗时，血清学方法交叉反应较多。使用PCR法进行鉴定，具有极高的灵敏度和特异性，且检测时间短，只需数小时即可获得结果。

本试剂盒选取了16S rRNA基因进行PCR鉴定，引物经BLAST验证为特异性靶向爱德华支原体，与其他生物基因组无交叉反应。检测13种支原体和3种细菌，只有爱德华支原体产生特异性条带，其他菌株均没有扩增。从58个犬气管组织样品中检出14个阳性。可见本试剂盒对爱德华支原体有特异性，可用于检测和鉴定。

本产品仅供科学研究使用。

 

 

 

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