Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit features:

1, Specific detection of Mycoplasma mycoides filamentous subspecies, no cross-reaction with other biological genomes.

2, High sensitivity, detection limit equivalent to 8 genome/reaction.

3. DNA polymerase adopts hot start method. It can inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, can be used Check the effectiveness of the kit.

5, with UDG enzyme and dUTP, can Reduce residual DNA contamination.


Mycoplasma mycoplasma is a group of infectious mycoplasmas that includes six members: Mmc, MmmLC, MmmSC, Mcc and Mccp . Since MmmLC has been reclassified to Mmc, the only remaining member of the filamentous subspecies of Mycoplasma mycoides is MmmSC.


MmmSC was first discovered in 1898 and is a contagious bovine pleuropneumonia . Symptoms of CBPP include fever, anorexia, cough, and dyspnea. Pathological examination shows serum fibrinosis, interstitial pneumonia, and pulmonary osteonecrosis. It is a major threat to the cattle industry and can cause great economic losses in endemic areas. . Sometimes cattle do not show symptoms after being infected. At this time, the infected cattle can easily become the source of infection. Limited by the lack of small animal disease models, the pathogenic mechanism of MmmSC remains unclear, and polysaccharide antigens on its cell surface may be the causative factor. European strains in morbidity and lethalityThe rate is lower than that of African strains, which may be related to its less hydrogen peroxide release. In the early stages of the disease epidemic, acute and subacute onset are the main symptoms, and then the disease turns to chronic development.


When detecting Mycoplasma mycoides, serological methods often have cross-reactions and have low sensitivity. Low, and in vitro culture method is more time-consuming. PCR is a highly sensitive and specific detection method that only takes a few hours to obtain results, which can effectively make up for the shortcomings of the above two methods. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.


This kit compares the genomes of several members of the Mycoplasma mycoides family. , a transmembrane protein gene was selected as a target to specifically identify the filamentous subspecies of Mycoplasma mycoides. It was verified by BLAST that there was no cross-reaction with other biological genomes. This kit detects 27 MmmSC strains, 33 strains belonging to other members of the Mycoplasma mycoides family, and 14 other types of mycoplasmas that parasitize cattle. Only MmmSC produces specific signals.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1，   特异性检测丝状支原体丝状亚种，与其他生物基因组无交叉反应。

2，   灵敏度高，检测极限相当于8个基因组/反应。

3，   DNA聚合酶采用热启动方式，可抑制非特异性扩增，降低背景荧光。

4，   带有阳性对照样品，可用于检验试剂盒有效性。

5，   带有UDG酶和dUTP，可降低残留DNA的污染。

 

丝状支原体族（Mycoplasma Mycoides cluster）是一组具有传染性的支原体，包括六个成员： Mmc（Mycoplasma mycoides subsp. Capri.，丝状支原体山羊亚种），MmmLC (Mycoplasma mycoides subsp. mycoides Large Colony，丝状支原体丝状亚种大克隆，现已被重新归类至Mmc)，MmmSC (Mycoplasma mycoides subsp. mycoides Small Colony，丝状支原体丝状亚种小克隆) ，Mycoplasma leachii (旧称：Mycoplasma sp. bovine group 7, Mbg7)，Mcc (Mycoplasma capricolum subsp. Capricolum，山羊支原体山羊亚种)和Mccp（Mycoplasma capricolum subsp. capripneumoniae，山羊支原体山羊肺炎亚种)。由于MmmLC已被重新归类至Mmc，因此，丝状支原体丝状亚种（Mycoplasma mycoides subsp. mycoides，简称Mmm）的成员仅剩MmmSC。

MmmSC于1898年首次发现，是牛传染性胸膜肺炎（CBPP，一种严重的呼吸道传染病，又称牛肺疫）的致病菌。CBPP症状表现为发热、厌食、咳嗽、呼吸困难，病理检查有血清纤维蛋白化、间质性肺炎和肺部骨坏死等，是养牛业的主要威胁，在流行区域可造成很大的经济损失。有时牛感染后并不表现出症状，此时患牛易成为传染源。受限于缺乏小型动物的疾病模型，MmmSC致病机制仍不清楚，其细胞表面的多糖抗原可能是致病因素。欧洲菌株在发病率和致死率上低于非洲菌株，可能与其释放过氧化氢较少有关。在疾病流行的初期，以急性和亚急性发病为主，随后转向慢性发展。

检测丝状支原体时血清学方法常出现交叉反应，且灵敏度较低，而体外培养法又较为耗时。PCR是一种高灵敏度和高特异性的检测方法，仅需几个小时即可获得结果，可以有效弥补上述两种方法的不足。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒通过将几种丝状支原体族成员的基因组进行比较，选取一个跨膜蛋白基因作为靶点，特异性识别丝状支原体丝状亚种，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了27个MmmSC菌株，以及分属丝状支原体族其他各成员的33个菌株，14种寄生于牛的其他种类支原体，仅有MmmSC产生特异性信号。

 

 

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