Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma mycoides filamentous subspecies without cross-reaction with other biological genomes.

2. High sensitivity, with a detection limit equivalent to 7-13 CFU/reaction.

3. Use hot-start DNA polymerase to inhibit non-specific amplification and reduce background fluorescence.

4, with positive control sample, which can be used to test the effectiveness of the kit.

5, with ROX reference dye to help correct sampling errors and differences between tubes.

6, with UDG enzyme and dUTP, which can reduce the contamination of residual DNA.


Mycoplasma mycoides filamentous Subspecies small clone is a member of the Mycoplasma mycoides family. Because Mycoplasma mycoides subspecies large clone has been reclassified as Mycoplasma mycoides subspecies goat, therefore, Mycoplasma mycoides subsp. mycoides, referred to as Mmm) only MmmSC remains.


MmmSC was first discovered in 1898 and is a contagious bovine pleuropneumonia (CBPP, The causative bacterium of a severe respiratory infectious disease, also known as bovine plague, manifests as serum fibrinosis, interstitial pneumonia and pulmonary osteonecrosis. It is a major threat to the cattle industry and can cause serious consequences in endemic areas. Big economic losses. Limited by the lack of small animal disease models, its pathogenic mechanism remains unclear, and polysaccharide antigens on its cell surface may be the causative factor. The European strain has lower morbidity and fatality rates than the African strain, which may be related to its lower release of hydrogen peroxide. In the early stages of the disease epidemic, acute and subacute onset are the main symptoms, and then the disease turns to chronic development.


MmmSC is relatively easy to culture in vitro, using liquid or solid agar medium, depending on the background Biochemical and serological identification was performed based on physical availability, growth inhibition and immunofluorescence. The complement fixation test is currently the main identification method. It is suitable for detection in the acute development stage of the disease, but it is not suitable for detection in the early and chronic development stages of the disease. Moreover, the operation is complex, time-consuming and expensive, and it is difficult to obtain accurate and reliable continuous data. the result of. Agglutination assays are faster, but have lower sensitivity and specificity. Western blotting and ELISA methods are prone to cross-reactivity, are complex to operate, and take a long time. PCR is the fastest and most efficient method, with higher sensitivity and greater specificity, and results can be obtained in just a few hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.


Since other PCR methods select target regions with little variation, This kit compares the genome of MmmSC with the most closely related MmmLC, selects a region with high variation as a target, and specifically identifies MmmSC. It has been verified by BLAST that there is no cross-reaction with other biological genomes. This kit detected a total of 36 strains of 8 mycoplasma species with close distribution range or close genetic relationship, and no non-specific signals were found.

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测丝状支原体丝状亚种，与其他生物基因组没有交叉反应。

2， 灵敏度高，检测极限相当于7-13个CFU/反应。

3， 采用热启动DNA聚合酶，可抑制非特异性扩增，降低背景荧光。

4， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

5， 带有ROX参比染料，帮助校正加样误差和管间差异。

6， 带有UDG酶和dUTP，可降低残留DNA的污染。

 

丝状支原体丝状亚种小克隆（Mycoplasma mycoides subsp. mycoides SC，简称MmmSC）是丝状支原体族的一员。由于丝状支原体丝状亚种大克隆（Mycoplasma mycoides subsp. mycoides LC，简称MmmLC）已被重新归类为丝状支原体山羊亚种（Mycoplasma mycoides subsp. capricolum）,因此，丝状支原体丝状亚种（Mycoplasma mycoides subsp. mycoides，简称Mmm）仅剩MmmSC。

MmmSC于1898年首次发现，是牛传染性胸膜肺炎（CBPP，一种严重的呼吸道传染病，又称牛肺疫）的致病菌，表现为血清纤维蛋白化、间质性肺炎和肺部骨坏死，是养牛业的主要威胁，在流行区域可造成很大的经济损失。受限于缺乏小型动物的疾病模型，其致病机制仍不清楚，其细胞表面的多糖抗原可能是致病因素。欧洲菌株在发病率和致死率上低于非洲菌株，可能与其释放过氧化氢较少有关。在疾病流行的初期，以急性和亚急性发病为主，随后转向慢性发展。

MmmSC体外培养较为容易，使用液体或固体琼脂培养基，根据底物利用度、生长抑制和免疫荧光等进行生化和血清学鉴定。补体结合实验是目前的主要鉴定方法，适合在疾病急性发展阶段进行检测，但不太适合在疾病早期和慢性发展阶段的检测，而且操作复杂，耗时且昂贵，很难精确且连续的获取可靠的结果。凝集测定实验比较快速，但是灵敏度和特异性较低。免疫印迹和ELISA方法则容易出现交叉反应，且操作复杂，耗时较长。PCR是最快速和最有效的方法，有更高的灵敏度和更强的特异性，仅需几个小时即可获得结果。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

由于其他的PCR方法多选择一些变异很少的靶点区域，本试剂盒通过将MmmSC与亲缘关系最近的MmmLC的基因组进行比较，选取一段变异较多的区域作为靶点，特异性识别MmmSC，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了分布范围或亲缘关系较近的8种支原体共36个菌株，未发现非特异信号。

 

 

 

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