Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit features:

1. Specific detection of Mycoplasma Leachii, unlike other biological genomes Cross-reactivity.

2. High sensitivity, with the lowest detection limit equivalent to 10 genomes/reaction.

3, with positive control sample, which can be used to test the effectiveness of the kit.

4, with ROX reference dye to help correct sampling errors and differences between tubes.

5, using hot-start DNA polymerase, which can inhibit non-specific amplification and reduce background fluorescence.

6, with UDG enzyme and dUTP, which can reduce the contamination of residual DNA.

Mycoplasma mycoides is a group of infectious mycoplasmas, including six members: Mmc, MmmLC, MmmSC, Mcc, Mccp and Mycoplasma leachii . These members cause a variety of diseases in ruminants ranging from mild to severe and fatal.


Mycoplasma Leachii was originally bred from cattle in 1963 Isolated from synovial fluid, it can cause arthritis, mastitis and calf pneumonia. It is a facultative anaerobic bacterium. It grows rapidly and can cause turbidity in liquid culture media or the formation of large omelette-shaped colonies on solid culture media. Sometimes the diameter of the colony can reach 2 mm. It can metabolize glucose but cannot utilize arginine and urea. It can be distinguished from other members of the Mycoplasma mycoides family in that it can metabolize trehalose but cannot metabolize mannose and glucosamine. According to the results of DNA hybridization experiments and protein sequence analysis, its genome is 85% homologous to MmmSC, and is located in the middle of Mycoplasma mycoides and Mycoplasma capricolum in terms of classification. It is similar to both in terms of metabolic pathways, antigenic properties and related diseases. the difference. During serological analysis, Mycoplasma Leachii often cross-reacts with other members of the Mycoplasma mycoides family, causing great interference in the diagnosis of infectious pleuropneumonia in cattle and sheep.


Mycoplasma Leachii can be cultured in vitro and serum Scientific methods are used for identification, but culture methods are more time-consuming and serological methods have more cross-reactions. The PCR method is used for identification, which has extremely high sensitivity and specificity, and the detection time is short, and the results can be obtained in just a few hours. Compared with the ordinary PCR method, the quantitative PCR method can not only accurately quantify, but also be more convenient to operate and less affected by environmental pollution.


This kit selects a membrane lipoprotein gene with a conserved sequence as the target. Specific recognitionMycoplasma Leachii, verified by BLAST, has no cross-reactivity with other biological genomes. This kit detects a total of 68 strains of each member of the Mycoplasma mycoides family, as well as 7 other mycoplasma species and 21 bacterial species with similar distribution ranges, with only 7Mycoplasma Lachii Thestrain produces a specific signal.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1，特异性检测Mycoplasma Leachii，与其他生物基因组没有交叉反应。

2，灵敏度高，最低检测极限相当于10个基因组/反应。 

3，带有阳性对照样品（组分C），可用于检验试剂盒有效性。

4，带有ROX参比染料，帮助校正加样误差和管间差异。

5，采用热启动DNA聚合酶，可抑制非特异性扩增，降低背景荧光。

6，带有UDG酶和dUTP，可降低残留DNA的污染。

 

丝状支原体族（Mycoplasma Mycoides cluster）是一组具有传染性的支原体，包括六个成员： Mmc（Mycoplasma mycoides subsp. Capri）、MmmLC (Mycoplasma mycoides subsp. mycoides Large Colony，现已被重新归类至Mmc) 、MmmSC (Mycoplasma mycoides subsp. mycoides Small Colony)、Mcc (Mycoplasma capricolum subsp. capricolum)、Mccp（Mycoplasma capricolum subsp. capripneumoniae)和Mycoplasma leachii (即Mycoplasma sp. bovine group 7, Mbg7)。这些成员在反刍动物中引起从较温和到严重致死的多种疾病。

Mycoplasma Leachii最初是在1963年从牛关节液中分离出来，可引起关节炎、乳腺炎和小牛肺炎，属于兼性厌氧菌；其生长迅速，可引起液体培养基浑浊，或在固体培养基上形成大的煎蛋状菌落，有时菌落直径可达2毫米，可代谢葡萄糖，不能利用精氨酸和尿素，可与其他丝状支原体族成员相区分的特点是其能代谢海藻糖但不能代谢甘露糖及葡糖胺。根据DNA杂交实验和蛋白序列分析结果，其基因组与MmmSC同源度为85%，分类上位于丝状支原体和山羊支原体的中间位置，在代谢途径、抗原特性和关联疾病上均与二者有所区别。在血清学分析时，Mycoplasma Leachii常与丝状支原体族其他成员发生交叉反应，对牛和羊的传染性胸膜肺炎的诊断造成很大干扰。

Mycoplasma Leachii可以用体外培养法和血清学方法进行鉴定，但培养法较为耗时，血清学方法交叉反应较多。使用PCR法进行鉴定，具有极高的灵敏度和特异性，且检测时间短，只需数小时即可获得结果。定量PCR法与普通PCR法相比，不仅可以精确定量，而且操作更为方便，更少受环境污染的影响。

本试剂盒选取一个序列保守的膜脂蛋白基因作为靶点，特异性识别Mycoplasma Leachii，经BLAST验证，与其他生物基因组没有交叉反应。本试剂盒检测了丝状支原体族的各成员共68个菌株，以及分布范围相似的7种其他支原体和21种细菌，仅有7个Mycoplasma Lachii菌株产生特异信号。

 

 

 

 

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