Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Ureaplasma Diversum was first isolated in 1969 and was initially defined as a non-pathogenic species, but it has recently been shown to cause damage to cattle tissue Damage to cells and organs. Ureaplasma differentialis is commonly found in the reproductive tract of cattle and is associated with major reproductive organ diseases in these animals. Infected cows may present with infertility, placentitis, fetal calf alveolitis and abortion or birth of weak calves, while in bulls it may cause low sperm motility, seminal vesiculitis and epididymitis. Nonetheless, the relationship between Ureaplasma difficile and reproductive abnormalities in cattle remains controversial, mainly because of the high proportion of positive detection rates also in normally breeding animals. Ureaplasma difficile is a facultative intracellular microorganism that can be detected both intracellularly and adherent to cell surfaces. Its invasion of bovine sperm has been associated with low sperm motility, suggesting that it may lead to the death of these cells. It can also induce TNF-α production in the uterus of experimentally infected mice, suggesting that it may significantly alter the homeostasis of the uterine microenvironment. The molecular mechanisms by which Ureaplasma species exert their virulence and pathogenicity on these cells and tissues are mostly unknown.


In vitro culture Both method and PCR method can be used to detect differential Ureaplasma. In vitro culture method is a classic detection method, but it takes a long time. The use of PCR method has the advantages of high sensitivity and strong specificity, and the detection time is short, and the results can be obtained in just a few hours.


The differential Ureaplasma PCR detection kit selects a sequence of the 16S rRNA gene PCR identification was carried out, and the primers were verified by BLAST to specifically target Ureaplasma difficile and had no cross-reaction with the genomes of other organisms. This kit was used to detect 5 kinds of bovine parasitic mycoplasma, and only Ureaplasma differential produced specific amplification bands; and the results of vaginal swab detection of 168 cattle with reproductive abnormalities showed that 64 were positive, with a sensitivity of Higher than in vitro culture method. It can be seen that this kit is species-specific and can be used for the identification and detection of differential Ureaplasma.


This kit is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

差异脲原体（Ureaplasma Diversum）1969年首次被分离出来，最初被定义为非致病性物种，但是最近已证明它会对牛组织细胞和器官造成损伤。 差异脲原体常见于牛的生殖道，与这些动物的主要生殖器官疾病有关。受感染的母牛可能出现不孕、胎盘炎、胎牛肺泡炎和流产或弱小犊牛出生的现象，而在公牛中它可能导致精子活力低下、精囊炎和附睾炎。尽管如此，差异脲原体和牛生殖异常之间的关系仍然存在争议，主要是因为在正常繁殖的动物中也有高比例的阳性检出率。差异脲原体是一种兼性细胞内微生物，既可以在细胞内检测到，也可以粘附在细胞表面。它对牛精子的侵入与精子活力低有关，表明它可能导致这些细胞的死亡。它也能诱导实验感染小鼠的子宫产生TNF-α，表明它可能显著改变子宫微环境的稳态。差异脲原体对这些细胞和组织发挥毒力和致病性的分子机制大多是未知的。

体外培养法和PCR法均可用于检测差异脲原体。体外培养法是经典的检测方法，但耗时较长，而使用PCR法则有灵敏度高和特异性强的优点，且检测时间短，仅需几个小时即可获得结果。

差异脲原体PCR检测试剂盒选取了16S rRNA基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向差异脲原体，与其他生物的基因组无交叉反应。使用本试剂盒检测了5种牛寄生性支原体，仅有差异脲原体产生特异性扩增条带；而对168个生殖异常牛的阴道拭子检测的结果显示，有64个呈阳性，灵敏度高于体外培养法。可见本试剂盒具有物种特异性，可用于差异脲原体的鉴定和检测。

 本试剂盒仅供科学研究使用。

 

 

 

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