Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 
None Mycoplasma cholestiformis is classified into the Mollicutes Class. There are 15 known species, including saprophytic bacteria and pathogenic bacteria. It can be found in animals, some Found in plants and insects. Acholeplasma Laidlawii was first discovered in 1936 and is relatively common in isolates from the lungs of calves suffering from enzootic pneumonia and the conjunctiva of sheep suffering from infectious ophthalmia, but it is not known its pathogenicity. In traditional biopharmaceutical operations, sterilization by filtration is often used. Acholeplasma reindeerii in the culture medium is a serious problem. It may contaminate bovine serum and may also be hidden in serum-free cell culture media products. In serum-free cell culture media, Acholeplasma reindeerii may survive and multiply for long periods at refrigerated temperatures or at room temperature.

Both in vitro culture methods and PCR methods can be used to detect Acholeplasma reinhardtii . In vitro culture method is a classic detection method, but it takes a long time. The use of PCR method has the advantages of high sensitivity and strong specificity, and the detection time is short, and the results can be obtained in just a few hours.

The Mycoplasma reindeerii PCR detection kit selects the 16S rRNA gene A sequence was identified by PCR, and the primers were verified by BLAST to specifically target Acholeplasma reindeerii, Acholeplasma hippikon, and Acholeplasma granulosa . This kit was used to detect 13 kinds of mycoplasma related to cell culture, and only Mycoplasma reindeerii produced specific bands; 98 cell culture samples confirmed to be contaminated by mycoplasma were tested, and 20 Mycoplasma reindeerii were identified. Samples free of Mycoplasma cholestae contamination. It can be seen that this kit can be used for the detection of acholesterolic Mycoplasma reindeerii in cell culture.

This kit is for scientific research use only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

无胆甾支原体（Acholeplasma）分类上属支原体（Mollicutes Class），已知的有15种，有腐生菌，也有致病菌，可在动物、一些植物和昆虫体内发现。莱氏无胆甾支原体（Acholeplasma Laidlawii）于1936年首次发现，在患地方性肺炎的牛犊肺和患传染性眼炎的绵羊结膜的分离物中较为常见，尚不清楚其致病性。在传统的生物制药操作中，通常使用过滤法除菌，培养基中的莱氏无胆甾支原体是一个严重的问题，它可能污染牛血清，也可能藏匿于无血清细胞培养基产品中。在无血清的细胞培养基中，莱氏无胆甾支原体可能会在冷藏温度或室温下长期存活并繁殖。

体外培养法和PCR法均可用于检测莱氏无胆甾支原体。体外培养法是经典的检测方法，但耗时较长，而使用PCR法则有灵敏度高和特异性强的优点，且检测时间短，仅需几个小时即可获得结果。

莱氏无胆甾支原体PCR检测试剂盒选取了16S rRNA基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向莱氏无胆甾支原体、马无胆甾支原体（Acholeplasma hippikon）、以及粒状无胆甾支原体（Acholeplasma granularum）。使用本试剂盒对13种与细胞培养有关的支原体进行检测，仅莱氏无胆甾支原体产生特异性条带；对98份确定有支原体污染的细胞培养样品进行检测，鉴别出20个被莱氏无胆甾支原体污染的样品。可见本试剂盒可用于细胞培养物中莱氏无胆甾支原体的检测。

 本试剂盒仅供科学研究使用。

 

 

 

 

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