Store in the dark at -20℃, transport at 2-8℃, valid for one year. Avoid repeated freezing and thawing.

 

 

Kit Features:

1. Specific detection of Mycoplasma pneumoniae in goats, without cross-reaction with other biological genomes.

2. The DNA polymerase used has the characteristics of strong anti-inhibition ability and good thermal stability; using hot start method, it can Inhibit non-specific amplification and reduce background fluorescence.

3. With positive control sample, which can be used to test the effectiveness of the kit.

4, with ROX reference dye to help correct sampling errors and differences between tubes.

5. With UDG enzyme and dUTP, it can reduce the contamination of residual DNA.

6. High sensitivity, the detection limit is equivalent to 100fg/reaction.

Mycoplasma capricolum subsp. goat Mycoplasma pneumoniae belongs to the Mycoplasma mycoides cluster), and its hosts include goats and sheep. Mccp can cause contagious goat pleuropneumonia, with symptoms including acute respiratory distress, cough, runny nose and severe fibrous pleuropneumonia; it can also cause goat mastitis, leading to mammary gland atrophy and loss of lactation ability.


Compared with culture and serological detection methods, PCRThe method has the advantages of fast speed, strong specificity and high sensitivity. Ordinary PCR experiments are cumbersome and cannot quantify target DNA. In contrast, real-time quantitative PCR can not only quantitatively analyze target DNA, but the experimental steps are also simpler and more sensitive.


Dye method Mycoplasma caprine pneumonia real-time quantitative PCR kit selects a membrane lipoprotein gene As a target, the primer was verified by BLAST to specifically target Mycoplasma caprico pneumoniae and has no cross-reactivity with the genomes of other organisms. This kit was used to detect a total of 60 strains of other members of the Mycoplasma mycoides family, as well as 7 species of mycoplasma and 21 species of bacteria with similar distribution ranges, and no non-specific signals were found.

 

 

 

-20℃避光保存，2-8℃运输，有效期一年。避免反复冻融。

 

 

试剂盒特点：

1， 特异性检测山羊肺炎支原体，与其他生物基因组没有交叉反应。

2， 使用的DNA聚合酶具有抗抑制能力强和热稳定性好的特点；采用热启动方式，可抑制非特异性扩增，降低背景荧光。

3， 带有阳性对照样品（组分C），可用于检验试剂盒有效性。

4， 带有ROX参比染料，帮助校正加样误差和管间差异。

5， 带有UDG酶和dUTP，可降低残留DNA的污染。

6， 灵敏度高，检测极限相当于100fg/反应。

 

山羊支原体亚种山羊肺炎支原体（Mycoplasma capricolum subsp. Capripneumoniae ，简称Mccp）属于丝状支原体族（Mycoplasma mycoides cluster），宿主包括山羊和绵羊。Mccp可引起传染性山羊胸膜肺炎，症状包括急性呼吸窘迫、咳嗽、流鼻涕和严重的纤维性胸膜肺炎；还可以引起山羊乳腺炎，导致乳腺萎缩，丧失泌乳能力。

与培养法和血清学检测方法相比，PCR法具有速度快、特异性强、灵敏度高的优势。普通PCR实验操作较为繁琐，无法对目标DNA进行定量，相比之下，实时定量PCR不仅可以对目标DNA进行定量分析，实验步骤也较为简单，且灵敏度更高。

染料法山羊肺炎支原体实时定量PCR试剂盒选取一个膜脂蛋白基因作为靶点，引物经BLAST验证为特异性靶向山羊肺炎支原体，与其他生物基因组无交叉反应。使用本试剂盒检测了丝状支原体族的其他成员共60个菌株，以及分布范围相似的7种支原体和21种细菌，均未发现非特异信号。

 

 

 

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