Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma mycoides is a group of infectious mycoplasmas, including six members: Mmc, MmmLC, Mcc, Mycoplasma leachii, MmmSC and Mccp . These members cause a variety of diseases in ruminants ranging from mild to severe and fatal.

 

Among members of the Mycoplasma mycoides family, there are differences in antigens, There are many similarities in genetic characteristics, and some species are difficult to identify using conventional methods, often causing confusion in diagnosis and taxonomy. The Mycoplasma mycoides family can be identified using in vitro culture and serological methods, but each has its own shortcomings: some species of Mycoplasma mycoides grow slowly, it takes three weeks to complete the isolation and identification work, and the sensitivity and specificity of the serological method are not ideal. The PCR method is used for identification, which has extremely high sensitivity and specificity, and the detection time is short, and the results can be obtained in just a few hours.

 

Mycoplasma mycoides PCR detection kit selected A gene fragment common to all members was identified by PCR. The primers were verified by BLAST to specifically target the Mycoplasma mycoplasma family. They cross-react with Mycoplasma feriruminatoris and do not produce specific amplification with the genomes of other organisms. Add strips. Mycoplasma feriruminatoris was isolated from the joint synovial fluid of wild goats. The living environment of Mycoplasma feriruminatoris is significantly different from that of Mycoplasma mycoides. When preparing samples, pay attention to avoid contamination and it will not be affected. This kit was used to detect 56 strains belonging to six members of the Mycoplasma mycoides family, as well as 10 other mycoplasmas and 3 types of bacteria that are distributed or closely related. The strains of the Mycoplasma mycoides family all produced specific bands. Other strains, including Mycoplasma putrefactive and Mycoplasma yeatsii, did not produce this band. It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma mycoides.

 

This kit is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

 

 丝状支原体族（Mycoplasma Mycoides cluster）是一组具有传染性的支原体，包括六个成员： Mmc（Mycoplasma mycoides subsp. Capri.）、MmmLC (Mycoplasma mycoides subsp. mycoides Large Colony，现已被重新归类至Mmc)、Mcc (Mycoplasma capricolum subsp. capricolum)、Mycoplasma leachii (Mycoplasma sp. bovine group 7, Mbg7)、MmmSC (Mycoplasma mycoides subsp. mycoides Small Colony)和Mccp（Mycoplasma capricolum subsp. capripneumoniae)。这些成员在反刍动物中引起从较温和到严重致死的多种疾病。

丝状支原体族成员之间在抗原、遗传特点方面有很多相似之处，有些种类用常规方法难以鉴别，在诊断和分类学上常引起混淆。丝状支原体族可以用体外培养法和血清学方法进行鉴定，但各有缺点：有些丝状支原体种类生长缓慢，需要花三个星期才能完成分离和鉴定工作，而血清学方法灵敏度和特异性均不理想。使用PCR法进行鉴定，具有极高的灵敏度和特异性，且检测时间短，只需数小时即可获得结果。

丝状支原体族PCR检测试剂盒选取了一段各成员共有的基因片段进行PCR鉴定，引物经BLAST验证为特异性靶向丝状支原体族，与和Mycoplasma feriruminatoris有交叉反应，与其他生物的基因组不产生特异性扩增条带。Mycoplasma feriruminatoris是从野生山羊的关节滑液中分离出来的，与丝状支原体族生活环境有明显差异，准备样品时注意避免污染就可不受影响。使用本试剂盒检测了分属丝状支原体族六个成员的56个菌株，以及其他10种分布或亲缘关系较近的支原体和3种细菌，丝状支原体族的菌株均产生特异性条带，而包括腐败支原体和Mycoplasma yeatsii在内的其他菌株均不产生该条带。可见本试剂盒具有种属特异性，可用于丝状支原体族的鉴定和检测。

本试剂盒仅供科学研究使用。

 

 

 

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