Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Hemotropic Mycoplasma is a type of parasite that lives on the surface of red blood cells. The bacteria, once named Hemobartonella and Eperythrozoon, were reclassified as mycoplasmas based on 16S rRNA gene sequence analysis. There are two types of haemophilic mycoplasmas that have been found in goats and sheep: Eperythrozoon Ovis and Candidatus Mycoplasma hemovis. Some people believe that these two are actually two 16S rRNA gene versions of a mycoplasma.

 

Epoerythrozoonosis is the disease The pathogen was once classified as Rickettsia. The evolutionary analysis of 16S rRNA showed that this species belongs to mycoplasma, so it was renamed Mycoplasma Ovis . The host is sheep, goat or deer. It is free in the plasma or attached to the surface of red blood cells, causing massive destruction of red blood cells, leading to anemia, jaundice, and the death of animals in severe cases of infection. Typically, infected animals appear weak, stunted and lag behind in the movements of the group. Young sheep die after getting sickThe mortality rate is high, and adult sheep often show chronic diseases or asymptomatic symptoms. Previously infected animals develop protective immunity. Eperythrozoon ovis has low pathogenicity and usually infects a large number of sheep with malnutrition, trace element deficiencies, helminthiasis and subacute poisoning and weak sheep, as well as sheep with reticuloendothelial system insufficiency . Blood inoculation can cause clinical symptoms and parasitemia.

 

There is no suitable in vitro culture of Eperythroblasts Methods, detection methods use Romanowsky-type detection of organisms attached to red blood cells, or acridine orange blood smear method, or serological methods. Epicytozoon parasitism often occurs at a low level and is transient. Its detection requires repeated examination of blood smears. Microscopic examination of blood smears has low detection sensitivity, and epierythrozoites cannot be detected in blood smears when the sheep is already anemic. Therefore, it is more convenient to use PCR method for detection, with higher sensitivity and short detection time, and the results can be obtained in only a few hours.

 

Epoerythroblast PCR detection kit selected A sequence of the 16S rRNA gene was identified by PCR, and the primers were verified by BLAST to specifically target Eperythrozoon melitensis and are only compatible with blood mycoplasmas such as Mycoplasma haemovis, Mycoplasma wenyonii, Mycoplasma erythrocervae, Mycoplasma haemocervae, Mycoplasma haemomeles There is cross-reactivity. Mycoplasma haemovis is very closely related to Eperythroblastoma sheep, and some believe they belong to the same species. This kit can detect both at the same time. Mycoplasma wenyonii is parasitic to cattle, Mycoplasma erythrocervae and Mycoplasma haemocervae are parasitic to deer, and Mycoplasma haemomeles is parasitic to badgers, both It is not parasitic on sheep, and interference can be eliminated by strict operation during blood sampling. This kit was used to test blood samples of 573 sheep, and 36 cases were positive for epierythrozoon, and the positive rate of young sheep was significantly higher than that of adults.Year of the Sheep . It can be seen that this kit is species-specific and can be used for the identification and detection of epierythroblasts.

 

This product is for Scientific research use.

 

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

嗜血支原体（Hemotropic Mycoplasma，或hemoplasma)是一类寄生于红细胞表面的细菌，曾被命名为血巴通氏体（Hemobartonella）和附红细胞体（Eperythrozoon），根据16S rRNA基因序列分析，被重新归类为支原体。在山羊和绵羊中已发现的嗜血支原体有两种：羊附红细胞体（Eperythrozoon Ovis）和Candidatus Mycoplasma hemovis。有观点认为这两者其实是一种支原体的两个16S rRNA基因版本。

羊附红细胞体是羊附红细胞体病的病原体，曾被归类为立克次氏体，通过对16S rRNA的进化分析表明该物种属于支原体，所以被重新命名为羊支原体（Mycoplasma Ovis），宿主为绵羊、山羊或鹿。它游离于血浆中或附着在红细胞表面，引起红细胞的大量破坏，进而导致贫血、黄疸，在感染严重的情况下可导致动物死亡。通常情况下，受感染的动物表现为虚弱，发育迟缓，落后于群体的行动。幼年羊发病后死亡率较高，成年羊往往表现为慢性疾病或无症状表现。以前感染过的动物会产生保护性免疫。羊附红细胞体致病力低，通常在营养不良、微量元素缺乏、蠕虫病和亚急性中毒及虚弱的绵羊，以及网状内皮系统机能不全(如行脾脏摘除术)的绵羊中，用大量感染血液接种,才能引起临诊症状和寄生虫血症。

羊附红细胞体尚无合适的体外培养方法，检测方法采用Romanowsky-type检测附着在红细胞上的生物体，或吖啶橙血涂片法，或血清学方法。羊附红细胞体寄生经常发生在一个较低的水平，呈暂时性。对其检测需要反复的检查血涂片。用显微镜观察血涂片的检测灵敏度较低，而且在羊已出现贫血的情况下无法用血涂片检测出羊附红细胞体。因此使用PCR法检测更为方便，且灵敏度更高，检测时间短，仅需几个小时即可获得结果。

羊附红细胞体PCR检测试剂盒选取了16S rRNA基因的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向羊附红细胞体，仅与Mycoplasma haemovis, Mycoplasma wenyonii, Mycoplasma erythrocervae, Mycoplasma haemocervae, Mycoplasma haemomeles等血液支原体有交叉反应。Mycoplasma haemovis与羊附红细胞体亲缘关系极近，有观点认为属于同一物种，本试剂盒可同时检测二者。Mycoplasma wenyonii寄生于牛，Mycoplasma erythrocervae和Mycoplasma haemocervae寄生于鹿，Mycoplasma haemomeles寄生于獾，均不是寄生于羊，在进行血液取样时严格操作即可排除干扰。使用本试剂盒检测了573只绵羊的血液样品，有36例呈羊附红细胞体阳性，且幼年羊（小于一岁）阳性率（10.6%）显著高于成年羊（0.41%）。可见本试剂盒具有物种特异性，可用于羊附红细胞体的鉴定和检测。

本产品仅供科学研究使用。

 

 

 

 

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