Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Sheep Mycoplasma pneumoniae is a respiratory pathogen that can infect sheep, including domestic sheep, domestic goats, bighorn sheep and goats. Mycoplasma ovis causes mycoplasma pneumonia, and can also induce lung infections together with many other bacteria. The pathogenesis of Mycoplasma ovis involves several aspects, such as: changing macrophage activity, inducing the ciliated epithelium of ruminants to produce suicide antibodies, inhibiting lymphocyte activity, etc. These are all important factors leading to diseases in sheep and other small ruminants. . M. ovis can also serve as a predictor of other bacterial and viral infections. Different strains of M. ovis are often found in a single animal, and different strains differ in virulence. Mycoplasma ovis can be found in the lungs, trachea and nasal cavities of small ruminants, and its detection can be carried out by in vitro culture, PCR identification and serological methods.

 

PCR is a fast and powerful detection method, which is similar to that of mycoplasma cultured in vitro. Detection sensitivity is similar or slightly stronger. Mycoplasma ovis is difficult to culture and takes about two weeks to grow. During this period, it is often easily covered up by other faster-growing mycoplasmas. Therefore, the PCR method has obvious advantages over in vitro culture.

 

Mycoplasma ovine pneumonia PCR detection kit targets the 16S rRNA gene for PCR identification, primers It has been verified by BLAST that it specifically targets Mycoplasma ovis pneumoniae and has only weak cross-reactivity with Mycoplasma bovoculi . The PCR products of the two are the same size. Using the nucleic acid restriction endonuclease HindIII to degrade the product, the two small fragments obtained are Mycoplasma ovis pneumoniae. The PCR product of Mycoplasma bovis will not be cut. Mycoplasma bovis and Mycoplasma ovis pneumoniae have different hosts, so they may not cause interference during use. This kit is compatible with Mycoplasma arginini, Mycoplasma bovis,There is no cross-reaction with nine common mycoplasma species in ruminants, including Acholeplasma Laidlawii . Therefore, this kit can be used for the identification and detection of Mycoplasma ovis pneumoniae.

 

This kit is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

绵羊肺炎支原体（Mycoplasma ovipneumoniae）是一种可以感染羊的呼吸道病原体，包括家养的绵羊，家养的山羊，大角羊和山羊等。  绵羊肺炎支原体引起羊支原体肺炎（又称羊传染性胸膜肺炎、非进行性肺炎），并且还可以与许多其他细菌一起诱发肺部感染。绵羊肺炎支原体的致病机制涉及几个方面，如：改变巨噬细胞活性、诱导反刍动物的纤毛上皮产生自杀抗体、抑制淋巴细胞活性等，这些都是导致绵羊和其他小反刍动物疾病的重要因素。绵羊肺炎支原体还可以作为其他细菌和病毒感染的预测因子。在一只动物中经常发现不同的绵羊肺炎支原体菌株，不同的菌株在毒力方面也不同。 绵羊肺炎支原体可以在小型反刍动物的肺，气管和鼻腔内发现，其检测可以通过体外培养、PCR鉴定和血清学方法进行。

PCR是一种快速而强大的检测手段,与支原体体外培养的检测敏感度相似或略强。绵羊肺炎支原体较难培养，生长大约需要两周的时间，在此期间，常有其他生长较快的支原体容易将其掩盖，因此，PCR法相比体外培养法具有明显的优势。

绵羊肺炎支原体PCR检测试剂盒针对16S rRNA基因进行PCR鉴定，引物经BLAST验证为特异性靶向绵羊肺炎支原体，仅与牛眼支原体（Mycoplasma bovoculi）有较弱的交叉反应。二者的PCR产物大小相同，用核酸限制性内切酶HindIII降解产物，得到两个小片段的为绵羊肺炎支原体，牛眼支原体的PCR产物不会被切断。牛眼支原体与绵羊肺炎支原体宿主不同，所以在使用中可不构成干扰。本试剂盒与包括精氨酸支原体（Mycoplasma Arginini）、牛支原体（Mycoplasma bovis）、和莱氏无胆甾支原体（Acholeplasma Laidlawii）等九种反刍动物常见的支原体无交叉反应。因此本试剂盒可用于绵羊肺炎支原体的鉴定和检测。

本试剂盒仅供科学研究使用。

 

 

 

 

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