Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Mycoplasma agalactiae is a mycoplasma A bacterium in the genus that does not have a cell wall surrounding its cell membrane and is therefore not affected by many common antibiotics such as penicillins or other beta-lactam antibiotics that target cell wall synthesis. Mycoplasma Agalactiae is the second mycoplasma known to humans after Mycoplasma mycoides and was first isolated in 1925.

 

Mycoplasma agalactiae is widely distributed globally and is responsible for 90% of goat and Nearly 100% of outbreaks of ovine infectious agalactia syndrome are caused by the pathogen, which affects both sexes. In addition to agalactia, it can also cause mastitis, arthritis and keratoconjunctivitis. The biochemical characteristics of Mycoplasma agalactiae are as follows: it lacks glucose and mannose metabolism and the ability to hydrolyze arginine and gelatin, has phosphatase activity, can reduce tetrazole under anaerobic and aerobic conditions, and has the ability to adsorb blood cells.

 

The detection of Mycoplasma agalactiae can be done through serological methods, or genetic methods, for detection. Mycoplasma bovis and Mycoplasma agalactiae are very similar in phenotype and genetics, and share a lot of common antigens and epitopes, so serological methods can distinguish them. It is more difficult; the two are also very similar in some metabolic pathways, which also limits the application of biochemical diagnostic methods.

 

The Mycoplasma agalactiae PCR Detection Kit selects an unknown functional gene for PCR identification , the primers were verified by BLAST to specifically target Mycoplasma agalactiae and did not produce specific amplification bands with the genomes of other organisms. This kit was used to detect 41 strains of Mycoplasma bovis or Mycoplasma agalactiae. Compared with three other pairs of primers for Mycoplasma agalactiae, this kit identified the most accurately and had the best specificity. It can be seen that this kit is suitable for the identification and detection of Mycoplasma agalactiae.

 

This kit is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

无乳支原体是支原体属中的一种细菌，其细胞膜周围没有细胞壁，因此不受许多常见的抗生素如青霉素或靶向细胞壁合成的其他β-内酰胺抗生素的影响。无乳支原体（Mycoplasma Agalactiae）是继丝状支原体之后第二个被人类所知的支原体，在1925年首次分离出来。

无乳支原体在全球范围内广泛分布，是造成90％山羊和接近100％绵羊传染性无乳症综合症爆发的病原体，雌雄均可感染，除了无乳症还可以引发乳腺炎、关节炎和角膜结膜炎。无乳支原体生物化学特征如下：缺乏葡萄糖和甘露糖代谢以及对精氨酸和明胶的水解能力，有磷酸酶活性，在厌氧和有氧条件下可还原四唑，有血球吸附能力。

对无乳支原体的检测，可以通过血清学方法（如免疫荧光法和免疫印迹法），或遗传学方法（如PCR扩增或寡核苷酸互补配对）， 进行检测。牛支原体（Mycoplasma bovis）和无乳支原体在表型和遗传基因上都有很高的相似性，且拥有非常多的共同抗原和表位，因此血清学方法区别二者较为困难；二者在一些代谢通路上也很相似，因此也限制了生化诊断方法的应用。

无乳支原体PCR检测试剂盒选取了一个未知功能基因进行PCR鉴定，引物经BLAST验证为特异性靶向无乳支原体，与其他生物的基因组不产生特异性扩增条带。使用本试剂盒检测了41个牛支原体或无乳支原体菌株，与另外三对无乳支原体引物平行对比，本试剂盒识别最为准确，特异性最好。可见本试剂盒适合用于无乳支原体的鉴定和检测。

本试剂盒仅供科学研究使用。

 

 

 

 

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