Store at -20°C in the dark and avoid repeated freezing and thawing when transported at 2-8°C. Without repeated freezing and thawing, the shelf life is one year.

 

Goat Mycoplasma subspecies Mycoplasma capricolum subsp. capripneumoniae belongs to the Mycoplasma mycoides cluster and can cause infectious diseases in goats. Pleuropneumonia is characterized by high pathogenicity and high mortality. It can also cause mastitis in goats, leading to mammary gland atrophy and loss of lactation ability. Hosts of M. capricopneumoniae include goats and sheep.

 

Mycoplasma caprine pneumonia grows slowly in vitro and is difficult to cultivate. Its colonies are similar to Other members of the Mycoplasma mycoides family are difficult to distinguish and often have cross-reactivity in serological tests. Therefore, using the PCR method for detection can not only achieve high sensitivity, but also avoid interference caused by other mycoplasmas.

 

The Mycoplasma goat pneumonia PCR detection kit selects genes related to anaerobic metabolism A sequence was identified by PCR, and the primers were verified by BLAST to specifically target Mycoplasma caprico pneumoniae and did not cross-react with the genomes of other organisms. This kit was used to detect 14 Mycoplasma cape pneumonia strains and 27 other closely related Mycoplasma mycoides strains, and only Mycoplasma cape pneumoniae produced an amplified band . This band can be cleaved by endonuclease ClaI into two fragments of 190 and 126 bp. This kit can specifically detect Mycoplasma capricopneumoniae even when mixed with other bacterial species . It can be seen that this kit is species-specific and can be used for the identification and detection of Mycoplasma caprine pneumonia. .

This kit is for scientific research only.

 

 

 

-20℃避光保存，2-8℃运输避免反复冻融。没有反复冻融的情况下，保质期一年。

 

 

山羊支原体亚种山羊肺炎支原体（Mycoplasma capricolum subsp. capripneumoniae）属于丝状支原体族（Mycoplasma mycoides cluster），可引起传染性山羊胸膜肺炎，该病有高致病性和高死亡率的特点，还可以引起山羊乳腺炎，导致乳腺萎缩，丧失泌乳能力。山羊肺炎支原体宿主包括山羊和绵羊。

山羊肺炎支原体在体外生长较慢，较难培养，其菌落与丝状支原体族其他成员不易区分，在血清学检测上也常有交叉反应。因此，使用PCR法进行检测，既可以达到很高的灵敏度，也可以避免其它支原体带来的干扰。

山羊肺炎支原体PCR检测试剂盒选取了基因组内与无氧代谢有关的一段序列进行PCR鉴定，引物经BLAST验证为特异性靶向山羊肺炎支原体，与其他生物的基因组不产生交叉反应。使用本试剂盒检测了14种山羊肺炎支原体菌株，以及27种血缘关系较近的其他丝状支原体菌株，仅有山羊肺炎支原体产生扩增条带（316bp）。该条带可被核酸内切酶ClaI切割为190和126 bp两个片段。即使在与其他菌种（包括其他丝状支原体菌株）混合的情况下，本试剂盒也可特异性检测出山羊肺炎支原体，可见本试剂盒具有物种特异性，可用于山羊肺炎支原体的鉴定和检测。

本试剂盒仅供科学研究使用。

 

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