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Quick Step Human Thyroxine antibody (TAb)elisa Kit
Quick Step Human Thyroxine antibody (TAb)elisa Kit
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  • NO.:QS2647Hu
    Sensitivity:0.4 ng/ml
    Assay range:1.2 ng/ml -65 ng/ml
    Standard:90ng/ml
    Sample Volume:10ul
    Wavelength:450nm
    Assay Time:60 minutes
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Details

 

Summary:

 

Purpose

 

Our Quick Step Human Thyroxine antibody (TAb)elisa Kit is to assay TAb levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to TAb. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)- conjugated antigen specific for TAb is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TAb and HRP conjugated TAb antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TAb. You can calculate the concentration of TAb in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS2647Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard

Concentration

OD Value

Average OD Value

Blank Well

0ng/ml

0.044

0.0480

0.052

S1

60ng/ml

1.999

1.9865

1.974

S2

40ng/ml

1.302

1.3115

1.321

S3

20ng/ml

0.687

0.6900

0.693

S4

10ng/ml

0.392

0.3855

0.379

S5

5ng/ml

0.202

0.2030

0.204 

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