Summary:

 

Purpose

 

Our Quick Step Human Serum-derived  Hyaluronan-Associated Protein (SHAP)elisa Kit is to assay SHAP levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to SHAP. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for SHAP is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain SHAP and HRP conjugated SHAP antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of SHAP. You can calculate the concentration of SHAP in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3987Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/ml	0.043	0.0475
		0.052
S1	72ng/ml	1.988	1.9805
		1.973
S2	48ng/ml	1.302	1.3095
		1.317
S3	24ng/ml	0.692	0.7035
		0.715
S4	12ng/ml	0.377	0.3855
		0.394
S5	6ng/ml	0.202	0.1980
		0.194

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