Summary:

 

Purpose

 

Our Quick Step Human Hypoxia inducible factor- 1 ELISA Kit is to assay HIF-1 levels in Human serum, plasma, culture media or any biological fluid.

 

Principle

 

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HIF-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HIF-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HIF-1 and HRP conjugated HIF-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HIF-1. You can calculate the concentration of HIF-1 in the samples by comparing the OD of the samples to the standard curve.

 

Typical Data

 

The standard curve of QS3176Hu is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

 

 

 

Standard	Concentration	OD Value	Average OD Value
Blank Well	0ng/L	0.035	0.0385
		0.042
S1	1200ng/L	1.973	1.9790
		1.985
S2	800ng/L	1.327	1.3330
		1.339
S3	400ng/L	0.705	0.7105
		0.716
S4	200ng/L	0.393	0.3885
		0.384
S5	100ng/L	0.186	0.1895
		0.193

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