Chinese Name	尿嘧啶DNA糖苷酶（UNG酶/UDG酶）
Storage	-20 ℃/-70 ℃

 

 

Ungine DNA glycosidase (UNG enzyme) product introduces the ungine DNA glycosidase (UNG, UDG). Pyraine. UNG enzymes are not activated to RNA and are mainly used in the anti -pollution of PCR amplification products.

 

The principle of its role is based on: if DUTP replaces DTTP into DNA in the PCR reaction, a PCR amplifier product containing DU -base is formed. U -alkaline -based glycoside bonds to degrade the PCR amplification product. Specifications 1U/μL Storage buffer 20mm tris-HCL, 150mm naCl, 1mm EDTA, 1mmdtt, 0.05% Tween20, 50% Glycerol, pH8.0.

Reaging composition:

1, Ung 1u/μL

 

2, 10 × reaction buffer: 200mm Tris-HCl (pH8.0, 25 ° C), 100mm) NaCl, 10mm EDTA, 1mg/ML BSA. Under the condition of active definition of 37 ° C, 1 hour degradation of 1 μg of a single -chain DNA enzyme amount of 1 μg is 1 unit. The preservation conditions are kept at -20 ° C every day or weekly. Long-term storage can be stored at -70 ° C, but it must be strictly avoiding repeated freezing and melting when saving of -70 ° C.

 

Quality Control:

 

1. SDS-PAGE electrical purity is greater than 98%;

 

2. De degradation activity, differences between batch, stability;

 

3. After 3 minutes of processing at 37 ° C, 103 copy the following U -temporal containing U -temporal should be completely degraded and cannot produce amplification products;

 

4, non -exogenous nucleic acid enzyme activity, non -exogenous internal cutting, external cutting nuclei acidic acid Enzyme pollution.

 

1、Incubatetheproductfor2minat50℃；

 

2、InactivateUNGby Heating to 95 ° C for 5 min.

 

Note:

1. Avoid multiple frozen melting, do not expose to a greater temperature fluctuation. Temperature fluctuations have greatly affected the stability of the product.

2. UNG enzymes can clear the PC contained in Dutp before the PCR reactionR product to avoid false positive results caused by pollution;

3. Different from the use efficiency of DUTP to DUTP and the sensitivity to UNG enzymes, if the UNG system is used, the detection sensitivity is used if the UNG system is used to cause detection sensitivity.Decrease, adjust the reaction system for adjustment and optimization;

4. Use a dedicated area and pipette before and after amplification, wear gloves and replace it frequently. After the PCR reaction is completed, do not turn on the reaction tube.Reduce the pollution of samples to the maximum reduction of PCR products.

 

Remarks: Product information may be optimized and upgraded.Please refer to the actual label information.

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