Chinese Name

蛋白酶抑制剂混合物(His-Tag蛋白纯化用, 100X)

 

 

 

Product description:

Cell or tissue extract contains many endogenous proteases, phosphates, etc., which can easily lead to protein degradation or decoration in the extract. It affects subsequent protein detection. Therefore, adding proper proteases, phosphates and other inhibitors to the extract is an effective way to prevent protein degradation and decorative modification. This product is used for HIS-TAG protein purified protease inhibitor mixture, which contains broad-spectrum lingine, cysteine \u200b\u200band acidic protease inhibitors, and amino peptidase inhibitors. In the ratio of 1: 100, the protease inhibitor mixture (HIS-TAG protein is purified, 100x) is used in the cracking solution to purify His-TAG protein and effectively suppress protein degradation.

Scope of application:

Various protease activity in HIS-TAG protein purification, such as lingicine, amino peptide, cysteine Proteinase, suline, and citizenin, metal protease, etc.

How to use:

Mixture of protease inhibitors (bacterial extraction, 100X), add 1: 100 ratio to cracking at the ratio of 1: 100, respectively In the liquid, you can use it after mixing. The cracking solution containing protease phosphatase inhibitors should be available for existing, and it should not be prepared for subsequent use after freezing.

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