Product Name	F(ab')₂ Fragment Goat Anti-rabbit IgG H&L / Biotin
Chinese Name	生物素标记的F(ab)2片段羊抗兔IgG H&L抗体
Alias	F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (Biotin); F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/Biotin; F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (Biotin); Biotin AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L);
Immunogen Species	Goat
Clonality	Polyclonal
React Species	Rabbit,
Applications	IHC-P=1:400-800  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
immunogen	Native Rabbit IgG
Lsotype	IgG
Purification	affinity purified by Protein G, nonspecific adsorbed
Buffer Solution	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
Storage	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Product Detail	Immunoglobulin G (IgG) is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ACE2) Polyclonal Antibody, Unconjugated (SL46001R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL60295G-Biotin 1:1000) instructionsand DAB staining.

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