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Product Name Crotonyl-Histone H2B (Lys11) Chinese Name Immunogen Species Rabbit Clonality Monoclonal Clone NO. A10A2 React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-1:2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The nucleus Form Liquid Concentration 1mg/ml Lsotype IgG Purification Affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 组蛋白经过各种酶催化的修饰,包括乙酰化,甲基化,磷酸化,Ubiquitin化等。赖氨酸巴豆酰化是新发现的一种控制染色体结构和基因转录的可逆修饰。赖氨酸巴豆酰化在从蠕虫到人的真核细胞中高度保守。组蛋白赖氨酸巴豆酰化独特的结构和基因组定位表明,它在机制和功能上不同于组蛋白赖氨酸乙酰化。具体地说,在人类体细胞和兔雄性生殖细胞基因组中,组蛋白巴豆酰化标志着活性启动子或潜在增强子。组蛋白H2B在Lys11位点的巴豆酰化可能在表观遗传调控中发挥重要作用,包括染色质重塑和DNA转录调控等。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Rat kidney, 5: Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: (-) HeLa, (+) HeLa+ crotonic acid (100 ng/ml, 18hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 kDa
Observed MW: 14 kDa
Tissue: Human breast
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60207R
Tissue: Mouse small intestine
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60207R
Tissue: Rat liver
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Rabbit) (sp-0023)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60207R
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