Product Name	Acetyl-Histone H3 (Lys27)
Chinese Name
Product Type	Acetylated anti
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	F11E3
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-1:2000 IHC-P=1:100-500 ICC=1:50-1:200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Antigen affinity purification
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	组蛋白翻译后修饰（PTMs）是Epigenetics调控染色质结构的关键机制，被称为“组蛋白密码”。组蛋白上的PTMs包括乙酰化、甲基化、磷酸化和新的酰化直接影响染色质对转录因子的可及性和其他表观遗传调控，改变基因组的稳定性，基因转录等。组蛋白乙酰化主要发生在核心组蛋白氨基末端的多个赖氨酸残基上，以响应各种刺激，在基因表达调控、DNA损伤修复、染色质动力学等方面发挥重要作用。大多数情况下，组蛋白H2A主要在Lys5、9、15和36位处发生乙酰化修饰。 H2B主要在Lys5、12、15、16和20位处发生乙酰化修饰。组蛋白H3主要在Lys4、9、14、18、23、27、56和79位处发生乙酰化修饰。组蛋白H4主要在Lys5、8、12、16和20位处发生乙酰化修饰。组蛋白乙酰转移酶（HATs）和组蛋白去乙酰化酶（HDACs）是主要的调节因子。
Product Picture	Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Mouse IgG H&L (HRP) Lysate: (-) HeLa, (+) HeLa+Sodium butyrate (30mM, 4hr) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 17 kDa Observed MW: 17 kDa Tissue: Human neuroblastoma Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:200 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Mouse)(sp-0024) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60051M Cell line: HeLa Fixative: 4% Paraformaldehyde Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:200 Primary incubation condition: 4°C overnight Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) Comment: Color red is the positive signal for SLM-60051M

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