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Product Name CDC45L Chinese Name 细胞分裂周期调控蛋白45抗体 Alias CDC 45; CDC45; CDC 45L; CDC45 (cell division cycle 45, S.cerevisiae homolog) like; CDC45 cell division cycle 45 like; CDC45 like; CDC45 related protein; CDC45_HUMAN; CDC45L2; Cell division control protein 45 homolog; Cell division cycle 45 like 2; Cell division cycle 45 like; PORC PI 1; PORC-PI-1. Research Area Cell biology Cyclin Cell differentiation Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Pig, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 66kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CDC45L: 485-566/566 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Function:
Required for initiation of chromosomal DNA replication.<
Subunit:
Associated with ORC2.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
Similarity:
Belongs to the CDC45 family.
SWISS:
O75419
Gene ID:
8318
Database links:Entrez Gene: 8318 Human
Entrez Gene: 12544 Mouse
Omim: 603465 Human
SwissProt: O75419 Human
SwissProt: Q9Z1X9 Mouse
Unigene: 474217 Human
Unigene: 1248 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDC45L) Polyclonal Antibody, Unconjugated (SL7785R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CDC45L Polyclonal Antibody, Unconjugated(SL7785R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation:Anti-CDC45L Polyclonal Antibody, Unconjugated(SL7785R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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