Product Name	Myosin light chain (phospho S20)
Chinese Name	磷酸化肌球蛋白调节多肽9(平滑肌亚型)抗体
Alias	MYL9 (phospho S20); p-MLC(Ser20); phospho-MLC(Ser20); p-Myosin light chain(Ser20); MYL9_HUMAN; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MLC2; MRLC1; MYRL2.
literatures	Specific References  (8)     |     SL7052R has been referenced in 8 publications. [IF=8.713] Zhao-Bo Luo. et al. Fecal transplant from myostatin deletion pigs positively impacts the gut-muscle axis. ELIFE. 2023; 12: e81858  WB ;  Mouse.   PubMed:37039469 [IF=5.396] Luqing Song. et al. Chlorogenic acid improves the intestinal barrier by relieving endoplasmic reticulum stress and inhibiting ROCK/MLCK signaling pathways.. Food Funct. 2022 Feb;:  WB ;  Human.   PubMed:10.1039/D1FO02662C [IF=4.192] Jinwen Yuan. et al. Reparative Effects of Ethanol-Induced Intestinal Barrier Injury by Flavonoid Luteolin via MAPK/NF-κB/MLCK and Nrf2 Signaling Pathways. J Agr Food Chem. 2021;XXXX(XXX):XXX-XXX  WB ;  Human.   PubMed:33749262 [IF=3.82] Ikemura, S., et al. "Preventive effects of the anti-vasospasm agent via the regulation of the Rho-kinase pathway on the development of steroid-induced osteonecrosis in rabbits." Bone (2013).  WB ;  Rabbit.   PubMed:23313282 [IF=3.56] Hoj, Jacob P., et al. "Cellular contractility changes are sufficient to drive epithelial scattering." Experimental Cell Research (2014).  WB ;  Dog.   PubMed:24780819 [IF=3.241] Su H et al. cis 9, trans 11, but not trans 10, cis 12 CLA isomer, impairs intestinal epithelial barrier function in IPEC-J2 cells and mice through activation of GPR120-[Ca2+]i and the MLCK signaling pathway. Food Funct. 2020 Apr 30;11(4):3657-3667.  WB ;  pig.   PubMed:32296804 [IF=3.118] Zhuang S et al. Rhein protects against barrier disruption and inhibits inflammation in intestinal epithelial cells. Int Immunopharmacol. 2019 Jun;71:321-327.  WB ;  Rat.   PubMed:30952096 [IF=1.06] Li, Wei, et al. "Tanshinone II a protects against liopolysaccharides-induced endothelial cell injury via Rho/Rho kinase pathway." Chinese Journal of Integrative Medicine 20 (2014): 216-223.  WB ;  Human.   PubMed:24615214
Product Type	Phosphorylated anti
Research Area	Cardiovascular  Signal transduction  Stem cells  Cytoskeleton  The cell membrane蛋白
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	20kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human MYL9 around the phosphorylation site of Ser20: AT(p-S)NV
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Subunit: Myosin is an hexamer of 2 heavy chains and 4 light chains. Tissue Specificity: Smooth muscle tissues and in some, but not all, nonmuscle cells. Similarity: Contains 3 EF-hand domains. SWISS: P24844 Gene ID: 10398 Database links: Entrez Gene: 10398 Human Entrez Gene: 98932 Mouse Entrez Gene: 296313 Rat Omim: 609905 Human SwissProt: P20689 Human SwissProt: P24844 Human SwissProt: Q9CQ19 Mouse SwissProt: Q64122 Rat Unigene: 504687 Human Unigene: 271770 Mouse Unigene: 228729 Rat Unigene: 6870 Rat
Product Picture	Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Myosin light chain (phospho S20) (SL7052R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kD Observed band size: 20 kD Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(SL7052R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(SL7052R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(SL7052R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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