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Product Name Frizzled 2 Chinese Name Wnt信号受体FZD2蛋白抗体 Alias Frizzled homolog 2; Frizzled-2; Frizzled2; Fz 2; Fz-2; Fz2; FZD 2; FZD2; FZD2_HUMAN; FzE 2; FzE2; hFz 2; hFz2. Research Area Tumour Cell biology Signal transduction Stem cells transcriptional regulatory factor G protein-coupled receptor Cell Surface Molecule G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 60kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Frizzled 2: 61-160/565 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins (FRPs) have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. Expression of frizzled-2 can be observed in the fetal kidney and lung and in the adult ovary and colon. The Wnt/cGMP/Ca2+ pathway is mediated by frizzled-2 . It binds Wnt proteins and signals by activating the release of stored calcium. Expression of frizzled-2 is regulated by Angiotensin II. Activated frizzled-2 suppresses the activity of protein kinase G, and activates NFAT-dependent transcription, the phosphatidylinositol pathway and calcium sensitive enzymes.
Function:
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Subcellular Location:
Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.
Post-translational modifications:
Ubiquitinated by ZNRF3, leading to its degradation by the proteasome (By similarity).
Similarity:
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
SWISS:
Q14332
Gene ID:
2535
Database links:Entrez Gene: 2535 Human
Entrez Gene: 57265 Mouse
Omim: 600667 Human
SwissProt: Q14332 Human
SwissProt: Q9JIP6 Mouse
Unigene: 142912 Human
Unigene: 597264 Human
Unigene: 36416 Mouse
Unigene: 92324 Rat
Product Picture Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti-Frizzled 2 (SL6623R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
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