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Product Name PRSS10 Chinese Name 丝氨酸蛋白酶10抗体 Alias Epitheliasin; PRSS10; Serine protease 10; TMPRSS2; TMPRSS2 ERG FUSION GENE, INCLUDED; TMPRSS2 ETV1 FUSION GENE, INCLUDED; TMPS2_HUMAN; Transmembrane protease serine 2 catalytic chain; Transmembrane protease, serine 2; Transmembrane protease, serine 2, EC 3.4.219; Transmembrane protease, serine 2, EC 3.4.219; D16Ertd61e. literatures Specific References (4) | SL6285R has been referenced in 4 publications.[IF=41.584] Daichi Yamasoba. et al. Virological characteristics of the SARS-CoV-2 Omicron BA.2 spike. CELL. 2022 May;: FC ; Human.[IF=5.923] Mohamed Elbadawy. et al. Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus. Int J Mol Sci. 2021 Jan;22(19):10763 IHC ; Bat.[IF=5.079] Gi-Cheol Park. et al. ACE2 and TMPRSS2 Immunolocalization and COVID-19-Related Thyroid Disorder. BIOLOGY-BASEL. 2022 May;11(5):697 IHC ; Human.[IF=3.355] Samuel James Brake. et al. SARS-CoV-2 (COVID-19) Adhesion Site Protein Upregulation in Small Airways, Type 2 Pneumocytes, and Alveolar Macrophages of Smokers and COPD – Possible Implications for Interstitial Fibrosis. Int J Chronic Obstr. 2022 Jan;17:101-115 IF,IHC ; Human.Research Area Tumour Cell biology Neurobiology Kinases and Phosphatases TumourCell biologyMaker Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 54kDa Cellular localization The cell membrane Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PRSS10: 301-400/492 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Extracellular proteases mediate the digestion of neighboring extracellular matrix components in initial tumor growth, allow desquamation of tumor cells into the surrounding environment, provide the basis for invasion of basement membranes in targeted metastatic organs and are required for release and activation of many growth and angiogenic factors (1,2). The TMPRSS2 gene encodes a 492 amino acid multimeric serine protease, which is mainly expressed in the mouse prostate and kidney, and is also expressed in the human small intestine, prostate, colon, stomach and salivary gland (3,4). TMPRSS2 contains several domains, including a serine protease domain of the S1 family, a scavenger receptor cysteine-rich domain of group A, an LDL receptor class A domain and a transmembrane domain (3). TMPRSS2 is expressed as a full length form and a cleaved protease domain (5) and its expression is increased by androgenic hormones. TMPRSS2 is also expressed in prostate carcinoma, suggesting that it may play a role in prostate carcinogenesis (6).
Function:
Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
Subcellular Location:
Cell membrane and Secreted. Activated by cleavage and secreted.
Tissue Specificity:
Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
Similarity:
Belongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain.
SWISS:
O15393
Gene ID:
7113
Database links:
Entrez Gene: 7113 Human
Entrez Gene: 50528 Mouse
Omim: 602060 Human
SwissProt: O15393 Human
SwissProt: Q9JIQ8 Mouse
Unigene: 439309 Human
Unigene: 276145 Mouse
Unigene: 81082 Rat
Product Picture Sample:
Lane 1: Du145 (Human) Cell Lysate at 30 ug
Lane 2: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-PRSS10 (SL6285R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PRSS10 Polyclonal Antibody, Unconjugated(SL6285R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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