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Product Name TCHP Chinese Name 抑癌蛋白TCHP抗体 Alias TCHP; Trichoplein keratin filament-binding protein; Tumor suppressor protein. literatures Specific References (1) | SL6113R has been referenced in 1 publications.[IF=6.126] Jessica Guerraet al. Long-Pentraxin 3 Affects Primary Cilium in Zebrafish Embryo and Cancer Cells via the FGF System. Cancers (Basel) . 2020 Jul 1;12(7):1756. WB ; zebrafish.Research Area Tumour immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TCHP: 401-498/498 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail A tumour suppressor protein which may act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery.
Function:
Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells.
Subunit:
Interacts specifically with keratin proteins including, KRT5, KRT6A, KRT8, KRT14, KRT16 and KRT18.
Subcellular Location:
Cytoplasm, cytoskeleton. Cytoplasm. Cell membrane. Mitochondrion.
Tissue Specificity:
Expressed at high levels in normal urothelial and breast epithelial cells. Also expressed in the smooth muscle and endothelial cells. Reduced expression seen in advanced bladder and breast carcinomas (at protein level). Ubiquitous. Expressed at highest levels in the heart, skeletal muscle, kidney, liver and testis.
Similarity:
Belongs to the TCHP family.
SWISS:
Q9BT92
Gene ID:
84260
Database links:Entrez Gene: 84260 Human
Omim: 612654 Human
SwissProt: Q9BT92 Human
Unigene: 410924 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TCHP) Polyclonal Antibody, Unconjugated (SL6113R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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