Product Name	phospho-MARK4 (Ser423)
Chinese Name	磷酸化丝氨酸/苏氨酸蛋白激酶MARK4抗体
Alias	MARK4(phospho Ser423); MARK4(phospho S423); MARK 4; MARK-4; MARK 4L; MARK L1; MARK4; MARK4 serine / threonine protein kinase; MARK4 serine/threonine protein kinase; MARK4_HUMAN; MARKL 1; MARKL1; MAP / microtubule affinity regulating kinase 4; MAP / microtubule affinity regulating kinase 4L; MAP/microtubule affinity regulating kinase 4; MAP/microtubule affinity regulating kinase like 1; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase-like 1; Putative protein product of Nbla00650; FLJ90097; KIAA1860; mKIAA1860.
Product Type	Phosphorylated anti
Research Area	Tumour  Signal transduction  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	75kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human MARK4 around the phosphorylation site of Ser423 : RH(p-S)DF
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]. SWISS: Q96L34 Gene ID: 57787 Database links: Entrez Gene: 57787 Human Entrez Gene: 232944 Mouse Entrez Gene: 680407 Rat Omim: 606495 Human SwissProt: Q96L34 Human SwissProt: Q8CIP4 Mouse Unigene: 34314 Human Unigene: 260504 Mouse
Product Picture	Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti- phospho-MARK4 (Ser423) (SL4660R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kD Observed band size: 77 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MARK4 (Ser423)) Polyclonal Antibody, Unconjugated (SL4660R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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