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Product Name Ube2B Chinese Name Ubiquitin激活酶2B抗体 Alias hHR6B; HR6B; RAD6 homolog B; RAD6B; UBE2B; UBE2B_HUMAN; Ubiquitin carrier protein B; Ubiquitin conjugating enzyme E2 B; Ubiquitin protein ligase B; Ubiquitin-conjugating enzyme E2 17 kDa; Ubiquitin-conjugating enzyme E2 B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B. Research Area Tumour immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Pig, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 17kDa Cellular localization The nucleus The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Ube2B: 1-100/152 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
Subunit:
Interacts with RAD18, UBR2 and WAC.
Subcellular Location:
Cell membrane (By similarity). Nucleus (By similarity). Note=In peripheral neurons, expressed both at the plasma membrane and in nuclei (By similarity).
Similarity:
Belongs to the ubiquitin-conjugating enzyme family.
SWISS:
P63146
Gene ID:
7320
Database links:Entrez Gene: 7320 Human
Entrez Gene: 22210 Mouse
Omim: 179095 Human
SwissProt: P63146 Human
SwissProt: P63147 Mouse
Unigene: 612096 Human
Unigene: 644421 Human
Unigene: 384918 Mouse
Unigene: 20766 Rat
Product Picture Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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