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Product Name BCMA Chinese Name Blymphocyte成熟因子(CD269)抗体 Alias B cell maturation antigen; B Cell Maturation Factor; B cell maturation protein; BCM; TNFRSF17; CD269; CD269 antigen; Tumor Necrosis Factor Receptor Member 17; Tumor necrosis factor receptor superfamily member 17; TNR17_HUMAN. Research Area Tumour Cell biology immunology transcriptional regulatory factor b-lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications ELISA=1:5000-10000 Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 20kDa Detection molecular weight 27 kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human BCMA: 12-110/184 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq]
Function:
Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
Subunit:
Associates with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6.
Subcellular Location:
Cell membrane; Single-pass type III membrane protein. Endomembrane system; Single-pass type III membrane protein.
Tissue Specificity:
Expressed in mature B-cells, but not in T-cells or monocytes.
DISEASE:
Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
Similarity:
Contains 1 TNFR-Cys repeat.
SWISS:
Q02223
Gene ID:
608
Database links:Entrez Gene: 608 Human
Omim: 109545 Human
SwissProt: Q02223 Human
Unigene: 2556 Human
Product Picture Black line : Positive blank control (Raji); Negative blank control (HL60)
Green line : Primary Antibody (Rabbit Anti-BCMA antibody (SL3850R) )
Orange line:Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488)
Raji(Positive)and HL60(Negative control)cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCMA Antibody(SL3850R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).Blank control: Raji (fixed with 2% paraformaldehyde (10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-BCMA antibody(SL3850R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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