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Product Name TMEFF2 Chinese Name 增生性息肉蛋白1抗体 Alias Tomoregulin-2; HPP 1; HPP1; Hyperplastic polyposis protein 1; TEFF 2; TEFF2; TENB 2; TENB2; Tomoregulin; TPEF; TR; Transmembrane protein containing epidermal growth factor and follistatin domains ; Transmembrane protein TENB2; Transmembrane protein with EGF like and two follistatin like domains 2; TEFF2_HUMAN. Research Area Tumour Cell biology immunology Neurobiology Growth factors and hormones Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 37kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TMEFF2: 301-374/374 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail TMEFF2 is a growth factor implicated in the progression of prostate cancer. It is highly expressed in the brain and may act as a survival factor for mesencephalic and hippocampal neurons.
Function:
May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
Subcellular Location:
Isoform 1: Membrane; Single-pass type I membrane protein (Potential).
Isoform 2: Membrane; Single-pass type I membrane protein (Potential).
Isoform 3: Secreted.
Tissue Specificity:
Highly expressed in adult and fetal brain, spinal cord and prostate. Expressed in all brain regions except the pituitary gland, with highest levels in amygdala and corpus callosum. Expressed in the pericryptal myofibroblasts and other stromal cells of normal colonic mucosa. Expressed in prostate carcinoma. Down-regulated in colorectal cancer. Present in Alzheimer disease plaques (at protein level). Isoform 3 is expressed weakly in testis and at high levels in normal and cancerous prostate.
Post-translational modifications:
N-glycosylated. Contains chondroitin sulfate glycosaminoglycans.
A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or proinflammatory cytokines such as TNFalpha, and is mediated by ADAM17.
Similarity:
Belongs to the tomoregulin family.
Contains 1 EGF-like domain.
Contains 2 Kazal-like domains.
SWISS:
Q9UIK5
Gene ID:
23671
Database links:
Entrez Gene: 23671 Human
Entrez Gene: 56363 Mouse
Omim: 605734 Human
SwissProt: Q9UIK5 Human
SwissProt: Q9QYM9 Mouse
Unigene: 144513 Human
Unigene: 245154 Mouse
Unigene: 445120 Mouse
Product Picture Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-TMEFF2 (SL3849R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (rat spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEFF2) Polyclonal Antibody, Unconjugated (SL3849R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEFF2) Polyclonal Antibody, Unconjugated (SL3849R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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