Product Name	ANO1
Chinese Name	Transmembrane protein16A/钙激活氯离子通道抗体
Alias	ANO 1; ANO1_HUMAN; Anoctamin 1; Anoctamin 1 calcium activated chloride channel; Anoctamin-1; Anoctamin1; Discovered on gastrointestinal stromal tumors protein 1; DOG 1; FLJ10261; Membrane protein; Oral cancer overexpressed 2; Oral cancer overexpressed protein 2; ORAOV 2; ORAOV2; TAOS 2; TAOS2; TMEM 16A; TMEM16A; Transmembrane protein 16A (eight membrane spanning domains); Transmembrane protein 16A; Tumor amplified and overexpressed sequence 2; Tumor-amplified and overexpressed sequence 2.
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	114kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DOG-1/TMEM16A: 151-250/986
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	TMEM16A (Transmembrane protein 16A) is an eight-transmembrane protein homologous to C12orf3, C11orf25 and FLJ34272 gene products and is amplified in various cancers. It acts as a calcium-activated chloride channel and is required for normal tracheal development. Function: Acts as a calcium-activated chloride channel. Required for normal tracheal development (By similarity). Subcellular Location: Cell membrane; Multi-pass membrane protein. Cytoplasm. Tissue Specificity: Broadly expressed with higher levels in liver and skeletal muscle. Similarity: Belongs to the anoctamin family. SWISS: Q5XXA6 Gene ID: 55107 Database links: Entrez Gene: 55107 Human Entrez Gene: 101772 Mouse Entrez Gene: 309135 Rat Omim: 610108 Human SwissProt: Q5XXA6 Human SwissProt: Q8BHY3 Mouse Unigene: 503074 Human Unigene: 26700 Mouse Unigene: 162509 Rat
Product Picture	Sample: Lane 1: Skeletal muscle (Mouse) Lysate at 40 ug Lane 2: Skeletal muscle (Rat) Lysate at 40 ug Lane 3: HepG2 (Human) Cell Lysate at 30 ug Lane 4: A673 (Human) Cell Lysate at 30 ug Lane 5: U-2OS (Human) Cell Lysate at 30 ug Lane 6: MKN45 (Human) Cell Lysate at 30 ug Primary: Anti-ANO1/TMEM16A (SL3794R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114-120 kD Observed band size: 120 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(SL3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TMEM16A Polyclonal Antibody, Unconjugated(SL3794R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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