Product Name	Phospho-Talin (Ser425)
Chinese Name	磷酸化踝蛋白抗体
Alias	Talin 1 (phospho S425); p-Talin 1 (phospho S425); TLN 1; ILWEQ; Talin 1; Talin; TLN; TLN1; TLN1_HUMAN.
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	280kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human Talin around the phosphorylation site of Ser425: SV(p-S)PK
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Function: Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Subunit: Binds with high affinity to VCL and with low affinity to integrins. Interacts with PTK2/FAK1. Interacts with PIP5K1C and NRAP. Interacts with LAYN. Interacts with SYNM. Subcellular Location: Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Note=Colocalizes with LAYN at the membrane ruffles. Similarity: Contains 1 FERM domain. Contains 1 I/LWEQ domain. SWISS: Q9Y490 Gene ID: 7094 Database links: Entrez Gene: 395194 Chicken Entrez Gene: 7094 Human Entrez Gene: 21894 Mouse Entrez Gene: 313494 Rat Omim: 186745 Human SwissProt: P54939 Chicken SwissProt: Q9Y490 Human SwissProt: P26039 Mouse Unigene: 471014 Human Unigene: 208601 Mouse Unigene: 7715 Rat TALIN-踝蛋白。是重要的Cytoskeleton肌动蛋白Binding protein，由两个270kDa的亚单位组成的反向平行的同源二聚体，与整合蛋白B尾部的结合诱导了整合蛋白胞外结构域构象的改变,起到了对整合蛋白的激活。
Product Picture	Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-Talin(Ser425) Polyclonal Antibody, Unconjugated(SL3719R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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