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Product Name HDAC6 Chinese Name 组蛋白去乙酰化酶6抗体 Alias HD 6; HD6; HDAC 6; Histone deacetylase 6; HD6; Histone deacetylase 6; JM 21; JM21; KIAA0901; FLJ16239; HDAC6_HUMAN. literatures Specific References (1) | SL2811R has been referenced in 1 publications.[IF=6.684] Huihua Kai. et al. LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6. Front Cell Dev Biol. 2021; 9: 701628 WB ; Human.Research Area Tumour Developmental biology Signal transduction Apoptosis transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 134kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HDAC6: 301-400/1215 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008].
Function:
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.
In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy.
Subunit:
Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15 (By similarity). Interacts with DDIT3/CHOP.
Subcellular Location:
Nucleus. Cytoplasm. Note=It is mainly cytoplasmic, where it is associated with microtubules.
Post-translational modifications:
Phosphorylated by AURKA.
Ubiquitinated. Its polyubiquitination however does not lead to its degradation.
Sumoylated in vitro.
Similarity:
Belongs to the histone deacetylase family. HD type 2 subfamily.
Contains 1 UBP-type zinc finger.
SWISS:
Q9UBN7
Gene ID:
10013
Database links:Entrez Gene: 10013 Human
Entrez Gene: 15185 Mouse
Omim: 300272 Human
SwissProt: Q9UBN7 Human
SwissProt: Q9Z2V5 Mouse
Unigene: 6764 Human
Unigene: 29854 Mouse
Unigene: 13453 Rat
组蛋白去乙酰化酶(HDACs)是一组在细胞染色质水平、通过诱导组蛋白去乙酰化来调控包括染色质重组、转录活化或抑制、细胞周期、Cell differentiation及Apoptosis等一系列生物学效应的酶,特别是与细胞活化后的基因转录表达调控有关。
HDAC6是一种比较独特的组蛋白去乙酰化酶,含有两个功能上相互独立的HDAC催化结构域。HDAC6可以去乙酰化组蛋白并抑制相关基因转录。
HDAC6可以和微管(microtuble)结合,可以去乙酰化tubulin,Hsp90和cortactin等。目前发现大量的蛋白可以被乙酰化修饰,因此HDAC等乙酰化修饰酶被认为在基因转录调控、Signal transduction、生长发育、分化凋亡、代谢性疾病和Tumour等多种生理病理过程中发挥重要作用。HDAC的抑制剂目前被认为是很有前景的Tumour治疗药物。
内源性HDAC6主要定位于cytoplasmic,与微管相结合并且是一个微管蛋白去乙酰化酶。HDAC6含有一个锌指结构域,该结构域可能和Ubiquitin化降解的调节有关。HDAC6可以和DHAC11相互作用。Product Picture Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-HDAC6 (SL2811R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kD
Observed band size: 134 kD
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDAC6) Polyclonal Antibody, Unconjugated (SL2811R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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