Product Name	CD32
Chinese Name	低亲和力免疫球蛋白γFc区受体II-a/Fc γ RII a抗体
Alias	CD 32; CD32A; CD32B; CD32C; CDw32; Fc gamma RII; low affinity II receptor; Fc gamma receptor II; Fc-GAMMA-RII; Fc[g]RII; IgG Fc receptor II; FCG 2; FCG2; FcgammaRII; FCGR 2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; FcgRII; Fcr-2; Fcr-3; FcRII a; FCRII; FcRII b; FcRII c; FcRIIC; IGFR 2; IGFR2; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17; MGC23887; MGC30032; FCG2C_HUMAN.
Research Area	Cell biology  immunology  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=0.2μg/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	31kDa
Detection molecular weight	40kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Fc gamma RII a: 201-300/317
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008] Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Subunit: Isoform IIB1 interacts with measles virus N protein. N protein is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response. Interacts with INPP5D/SHIP1. Interacts with FGR. Interacts with LYN. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. Post-translational modifications: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P12318 Gene ID: 2212 Database links: Entrez Gene: 2212 Human Entrez Gene: 2213 Human Entrez Gene: 9103 Human Entrez Gene: 116591 Rat Entrez Gene: 289211 Rat Omim: 146790 Human Omim: 604590 Human Omim: 612169 Human SwissProt: P12318 Human SwissProt: P31994 Human SwissProt: P31995 Human SwissProt: Q63203 Rat Unigene: 352642 Human Unigene: 154415 Rat
Product Picture	Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-CD32  (SL2573R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD Sample: K562 Cell Lysate at 30 ug Primary: Anti- CD32 (SL2573R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 33 kD Blank control: U937 (blue). Primary Antibody:Rabbit Anti-CD32 antibody(SL2573R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (SL2573R, 0.2μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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