Product Name	GTSE1
Chinese Name	G2期/S期应答相关蛋白1抗体
Alias	G2 and S phase-expressed protein 1; B99; B99 homolog; G 2 and S phase expressed 1; G two S phase expressed protein 1; GTSE 1; GTSE-1; GTSE1_HUMAN.
Research Area	Cell biology  immunology  Cyclin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	77kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GTSE1: 651-739/739
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis. [provided by RefSeq, Jul 2008] Function: May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression. Subcellular Location: Cytoplasm, cytoskeleton. Note=Associated with microtubules. Post-translational modifications: Phosphorylated in mitosis. Similarity: Sequence=CAQ09324.2; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=CAQ10552.2; Type=Erroneous initiation; Note=Translation N-terminally shortened. SWISS: Q9NYZ3 Gene ID: 51512 Database links: Entrez Gene: 51512 Human Omim: 607477 Human SwissProt: Q9NYZ3 Human
Product Picture	Sample: Hela(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug Primary: Anti- GTSE-1 (SL2516R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 75 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GTSE-1) Polyclonal Antibody, Unconjugated (SL2516R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: Human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GTSE-1 Polyclonal Antibody, Unconjugated(SL2516R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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