Product Name	LAMP2
Chinese Name	溶酶体相关膜蛋白2（CD107B）抗体
Alias	CD107 antigen-like family member B; CD107b; CD107b antigen; Igp110; Igp2; LAMP 2; LAMP 2C; LAMP B; LAMP-2; LAMP2_RAT; LAMPB; LGP110; Lysosomal associated membrane protein 2C; Lysosome associated membrane glycoprotein 2 precursor; Lysosome associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2; MAC3.
literatures	Specific References  (10)     |     SL2379R has been referenced in 10 publications. [IF=14.224] Ling Liu. et al. Polystyrene micro(nano)plastics damage the organelles of RBL-2H3 cells and promote MOAP-1 to induce apoptosis. J HAZARD MATER. 2022 Sep;438:129550  WB ;  Rat.   PubMed:35999725 [IF=6.048] Hao Liu. et al. Quercetin protects against palmitate-induced pancreatic β-cell apoptosis by restoring lysosomal function and autophagic flux. J NUTR BIOCHEM. 2022 May;:109060  WB,IF ;  Rat.   PubMed:35643286 [IF=6.025] Limei Yang. et al. The disruption of human trophoblast functions by autophagy activation through PI3K/AKT/mTOR pathway induced by exposure to titanium carbide (Ti3C2) MXene. FOOD CHEM TOXICOL. 2022 May;:113128  WB ;  Human.   PubMed:35569596 [IF=5.98] Luks, Lisanne, et al. "Novel insights into renal d-amino acid oxidase accumulation: propiverine changes DAAO localization and peroxisomal size in vivo." Archives of Toxicology (2016): 1-11.  IHC-P ;  Rat.   PubMed:26961980 [IF=5.23] Kameyama, Hirokazu, et al. "Iowa Mutant Apolipoprotein AI (ApoA-IIowa) Fibrils Target Lysosomes." Scientific Reports 6 (2016): 30391.  WB ;  Human.   PubMed:27464946 [IF=4.556] Woojin Na. et al. Aesculetin Inhibits Osteoclastic Bone Resorption through Blocking Ruffled Border Formation and Lysosomal Trafficking. Int J Mol Sci. 2020 Jan;21(22):8581  WB ;  Mouse.   PubMed:33203061 [IF=3.66] Iwao, Yasuhito, et al. "Liver atrophy after percutaneous transhepatic portal embolization occurs in two histological phases: Hepatocellular atrophy followed by apoptosis." World Journal of Hepatology 9.32 (2017): 1227.  IHC-P ;  Pig.   PubMed:29184609 [IF=3.274] Dong W et al. Polybrominated Diphenyl Ethers Quinone Induces NCOA4-mediated Ferritinophagy through Selectively Autophagic Degradation of Ferritin. Chem Res Toxicol. 2019 Nov 11.  WB ;  Rattus norvegicus.   PubMed:31687807 [IF=3.23] Sun, Qianqian, et al. "Factors that Affect Pancreatic Islet Cell Autophagy in Adult Rats: Evaluation of a Calorie-Restricted Diet and a High-Fat Diet."PLOS ONE 11.3 (2016): e0151104.  IHC-P ;  Rat.   PubMed:26963814 [IF=0] Iwao et al. Liver atrophy after percutaneous transhepatic portal embolization occurs in two histological phases: Hepatocellular atrophy followed by apoptosis. (2017) World.J.Hepatol. 9:1227-1238  IHC-P ;  Porcine.   PubMed:29184609
Research Area	Tumour  Cardiovascular  Cell biology  Signal transduction  Stem cells  The new supersedes the old  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Detection molecular weight	110 kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human LAMP2: 301-411/411
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by the LAMP2 gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands and is thought to play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of the gene produces two known products, LAMP2a and LAMP2b. Isoform LAMP2a is highly expressed in placenta, lung and liver and has low expression in brain and skeletal muscle. The isoform detected by this antibody, LAMP2b, is highly expressed in skeletal muscle less so in brain, placental, lung, kidney and pancreas and has very low expression in liver. Function: Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Post-translational modifications: O- and N-glycosylated; some of the N-glycans attached to Lamp-2 are polylactosaminoglycans. Similarity: Belongs to the LAMP family. SWISS: P13473 Gene ID: 3920 Database links: Entrez Gene: 3920 Human Entrez Gene: 16784 Mouse Omim: 309060 Human SwissProt: P13473 Human SwissProt: P17047 Mouse Unigene: 496684 Human Unigene: 486 Mouse
Product Picture	Sample: Lane 1: U251 (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: 293T (Human) Cell Lysate at 30 ug Lane 4: HL-60 (Human) Cell Lysate at 30 ug Lane 5: HUVEC (Human) Cell Lysate at 30 ug Lane 6: RAW264.7 (Mouse) Cell Lysate at 30 ug Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug Primary: Anti-LAMP2 (SL2379R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (SL2379R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (SL2379R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (SL2379R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (SL2379R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (SL2379R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LAMP2 Polyclonal Antibody, Unconjugated(SL2397R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LAMP2 Polyclonal Antibody, Unconjugated(SL2397R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control(blue):U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- LAMP2 antibody(SL2379R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Blank control(blue): HELA (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-LAMP2 Receptor beta antibody(SL2379R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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