Product Name	SPRED2
Chinese Name	SPRED2蛋白抗体
Alias	C79158; FLJ21897; FLJ31917; SPRE2_HUMAN; MGC163164; Spred 2; Sprouty protein with EVH 1 domain 2 related sequence; sprouty related EVH1 domain containing 2; Sprouty related protein with EVH 1 domain 2; zgc:77284.
Research Area	Cell biology  Signal transduction  Kinases and Phosphatases  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	47kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SPRED2 : 381-480/674
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008] Function: Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase. Subunit: Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers. Subcellular Location: Cell Membrane - peripheral membrane protein and Cytoplasmic. Tissue Specificity: Expressed in liver, skin, small intestine, salivary gland and prostate. Post-translational modifications: Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination. Ubiquitinated; leading to degradation by the proteasome. Similarity: Contains 1 KBD domain. Contains 1 SPR (sprouty) domain. Contains 1 WH1 domain. SWISS: Q7Z698 Gene ID: 200734 Database links: Entrez Gene: 200734 Human Entrez Gene: 114716 Mouse Entrez Gene: 305539 Rat Omim: 609292 Human SwissProt: Q7Z698 Human SwissProt: Q924S7 Mouse SwissProt: Q3C2P8 Rat Unigene: 59332 Human
Product Picture	Sample: Small inteatine (Mouse) Lysate at 40 ug Primary: Anti- SPRED2 (SL21897R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 47 kD Observed band size: 48 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPRED2) Polyclonal Antibody, Unconjugated (SL21897R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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