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Product Name ALPI Chinese Name 碱性磷酸酶(肠型)抗体 Alias PPBI_HUMAN; Intestinal-type alkaline phosphatase; EC:3.1.3.1; IAP; Intestinal alkaline phosphatase; alkaline phosphatase, intestinal; 肠道碱性磷酸酶; 肠碱性磷酸酶; 碱性磷酸酶,肠型; 肠型碱性磷酸酶; Research Area Tumour immunology Signal transduction Kinases and Phosphatases The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Human, Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 53kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ALPI: 351-450/528 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008]
Subunit:
Homodimer.
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
Similarity:
Belongs to the alkaline phosphatase family.
SWISS:
P09923
Gene ID:
248
Database links:Entrez Gene: 248 Human
Omim: 171740 Human
SwissProt: P09923 Human
Unigene: 284255 Human
Unigene: 37009 Human
Product Picture Sample:
Lane 1: Human LOVO cell lysates
Lane 2: Human FHC cell lysates
Lane 3: Human SW480 cell lysates
Primary: Anti-ALPI (SL20013R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 65 kDa
Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-Intestinal alkaline phosphatase antibody (SL20013R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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