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Product Name Rho guanine exchange factor 16 Chinese Name 鸟嘌呤交换因子16抗体 Alias ARHGEF 16; ARHGEF16; ARHGG_HUMAN; Ephexin 4; Ephexin-4; EPHEXIN4; GEF16; NBR; Putative Neuroblastoma Protein; Rho guanine exchange factor (GEF) 16; Rho guanine nucleotide exchange factor (GEF) 16; Rho guanine nucleotide exchange factor 16. Research Area Cell biology Signal transduction G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 80kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human RHEBL1: 151-250/709 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions. [provided by RefSeq, Jul 2008]
Function:
Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 and mediate activation of CDC42 by the viral protein HPV16 E6.
Subunit:
Interacts with ELMO2, EPHA2, RAC1 and RHOG; mediates activation of RAC1 by EPHA2. Interacts with TAX1BP3 (via PDZ domain). May interact with CDC42; stimulated by HPV16 E6.
Subcellular Location:
Cytoplasm.
Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
SWISS:
Q5VV41
Gene ID:
27237
Database links:Entrez Gene: 27237 Human
Entrez Gene: 230972 Mouse
SwissProt: Q5VV41 Human
SwissProt: Q3U5C8 Mouse
Unigene: 87435 Human
Unigene: 38481 Mouse
Product Picture Sample:Thymus (Mouse) Lysate at 40 ug
Primary: Anti-Rho guanine exchange factor 16(SL19870R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80kD
Observed band size: 80kD
Sample:Placenta (Mouse) Lysate at 40 ug
Primary: Anti-Rho guanine exchange factor 16(SL19870R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80kD
Observed band size: 80kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rho guanine exchange factor 16) Polyclonal Antibody, Unconjugated (SL19870R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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