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Product Name MRPL10 Chinese Name Mitochondrion核糖体蛋白L10抗体 Alias 39S ribosomal protein L10; 39S ribosomal protein L10 mitochondrial; 39S ribosomal protein L10 mitochondrial precursor; 39S ribosomal protein L8; L10mt; L8mt; MGC17973; mitochondrial; Mitochondrial ribosomal protein L10; MRP L10; MRP L8; MRP-L10; MRP-L8; MRPL 10; MRPL10; MRPL8; RM10_HUMAN; RPML8. Research Area Cell biology Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Cow, Horse, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 26kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MRPL10: 101-200/261 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]
Subcellular Location:
Mitochondrion.
Similarity:
Belongs to the ribosomal protein L10P family.
SWISS:
Q7Z7H8
Gene ID:
124995
Database links:Entrez Gene: 124995 Human
Entrez Gene: 107732 Mouse
Omim: 611825 Human
SwissProt: Q7Z7H8 Human
SwissProt: Q3TBW2 Mouse
Unigene: 347535 Human
Unigene: 208946 Mouse
Product Picture Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-MRPL10 (SL17768R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRPL10) Polyclonal Antibody, Unconjugated (SL17768R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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