TEL: +86 571 56623320 EMAIL: [email protected]
Product Name phospho-KIF22 (Ser427) Chinese Name 磷酸化驱动蛋白家族成员22抗体 Alias KIF22 (phospho S427); p-KIF22 (phospho S427); p-KID (Ser 427); A 328A3.2; KID; KIF 22; kif22; KIF22_HUMAN; Kinesin family member 22; Kinesin like 4; Kinesin like DNA binding protein; Kinesin like DNA binding protein pseudogene; Kinesin like protein 4; Kinesin like protein KIF22; Kinesin-like DNA-binding protein; Kinesin-like protein 4; Kinesin-like protein KIF22; KNSL 4; KNSL4; OBP 1; OBP 2; OBP; OBP1; OBP2; Origin of plasmid DNA replication binding protein; OriP binding protein; OTTHUMP00000123406. Product Type Phosphorylated anti Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Dog, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 73kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human KIF22 around the phosphorylation site of Ser427: KL(p-S)PL Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Function:
Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
Subcellular Location:
Nucleus. Cytoplasm > cytoskeleton.
Post-translational modifications:
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
SWISS:
Q14807
Gene ID:
3835
Database links:Entrez Gene: 3835 Human
Omim: 603213 Human
SwissProt: Q14807 Human
Unigene: 612151 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KIF22 (Ser427)) Polyclonal Antibody, Unconjugated (SL17050R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode