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Product Name phospho-TFRC (Ser24) Chinese Name 磷酸化转铁蛋白受体(CD71)抗体 Alias Transferrin Receptor (phospho S24); p-Transferrin Receptor (phospho S24); Transferrin receptor protein 1; Transferrin receptor; CD71; CD 71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TR; sTfR; Transferrin receptor (p90 CD71); TRFR; TFR1_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Signal transduction Stem cells transcriptional regulatory factor Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 85kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human Transferrin Receptor around the phosphorylation site of Ser24: RF(p-S)LA Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]
Function:
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
Subcellular Location:
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications:
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Similarity:
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
SWISS:
P02786
Gene ID:
7037
Database links:Entrez Gene: 7037 Human
Entrez Gene: 22042 Mouse
Omim: 190010 Human
SwissProt: P02786 Human
SwissProt: Q62351 Mouse
Unigene: 529618 Human
Unigene: 28683 Mouse
Product Picture Blank control (blue line): HL60(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-phospho-Transferrin Receptor (Ser24) antibody (SL16668R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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