Product Name	FMNL3
Chinese Name	FMNL3蛋白抗体
Alias	FHOD3; FMNL3_HUMAN； Formin homology 2 domain containing protein 3; Formin like protein 3; WBP3; WW domain binding protein 3.
literatures	Specific References  (2)     |     SL16151R has been referenced in 2 publications. [IF=4.966] Pan ZN et al. RAB7 GTPase regulates actin dynamics for DRP1‐mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J. 2020 Jul;34(7):9615-9627.  IP ;  Mouse.   PubMed:32472654 [IF=4.966] Pan Z et al. RAB7 GTPase regulates actin dynamics for DRP1‐mediated mitochondria function and spindle migration in Mouseoocyte meiosis. FASEB J . 2020 Jul;34(7):9615-9627.  WB ;  Mouse.   PubMed:32472654
Research Area	Cell biology  immunology  Signal transduction  Binding protein  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	117kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FMNL3: 951-1028/1028
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene contains a formin homology 2 domain and has high sequence identity to the mouse Wbp3 protein. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Function: FMNL3 is an actin-binding protein that contains a formin homology 2 domain. Subunit: Interacts with SRGAP2 (via SH3 domain). Subcellular Location: Cytoplasm. Note=Enriched in lamellipodia. Similarity: Belongs to the formin homology family. Contains 1 DAD (diaphanous autoregulatory) domain. Contains 1 FH2 (formin homology 2) domain. Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. SWISS: Q8IVF7 Gene ID: 91010 Database links: Entrez Gene: 91010 Human SwissProt: Q8IVF7 Human Unigene: 179838 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FMNL3) Polyclonal Antibody, Unconjugated (SL16151R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: Molt4. Primary Antibody (green line): Rabbit Anti- FMNL3/FITC Conjugated antibody (SL16151R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

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