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Product Name APOH Chinese Name 载LipoproteinH抗体 Alias Apolipoprotein H; Activated protein C binding protein; APC inhibitor; APOH; B2G1; beta 2 glycoprotein I; beta 2 glycoprotein I precursor; BG; OTTMUSP00000003033; APOH_HUMAN. literatures Specific References (2) | SL1570R has been referenced in 2 publications.[IF=38.104] Du Xing. et al. B cell-derived anti-beta 2 glycoprotein I antibody mediates hyperhomocysteinemia-aggravated hypertensive glomerular lesions by triggering ferroptosis. SIGNAL TRANSDUCT TAR. 2023 Mar;8(1):1-16 Other ; Mouse.[IF=4.543] Subhojit Paul. et al. Modulation of lung cytoskeletal remodeling, RXR based metabolic cascades and inflammation to achieve redox homeostasis during extended exposures to lowered pO 2. 2021 May 17 WB ; Human, Rat.Research Area Cardiovascular immunology Synthesis and Degradation glycoprotein Lipoprotein Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 36kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ApoH: 281-345/345 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Apolipoprotein H is expressed by placental trophoblast cells at high levels. Although the normal physiological role is not known, the protein appears to act as a co-factor for the binding of autoantibodies to phospholipids to trophoblasts, which is a process involved in the pathogenesis of recurrent miscarriage.
Function:
Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed by the liver and secreted in plasma.
Post-translational modifications:
N- and O-glycosylated. PubMed:6587378 also reports glycosylation on 'Asn-188' for their allele.
Similarity:
Contains 4 Sushi (CCP/SCR) domains.
SWISS:
P02749
Gene ID:
350
Database links:Entrez Gene: 350 Human
Omim: 138700 Human
SwissProt: P02749 Human
Unigene: 445358 Human
Unigene: 1824 Rat
载LipoproteinH(apolipoprotein D,apoH)又称为β2glycoproteinⅠ(β2-glycoprotein,β2-GI),是最初是在人血浆的高密度Lipoprotein中被分离出来的。apoH在结构上与其他类型的载Lipoprotein存在很大的差异,被归入脂肪促成素家族。Product Picture Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-APOH (SL 1570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35/50 kD
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti-APOH (SL 1570R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 35/50 kD
Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Liver (Rat) Lysate at 40 ug
Lane 3: Pancreas (Rat) Lysate at 40 ug
Primary: Anti-APOH (SL1570R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44-50 kD
Observed band size: 47 kD
Tissue/cell: human laryngeal cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOH Polyclonal Antibody, Unconjugated(SL1570R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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