Product Name	GLP-1R
Chinese Name	胰高血糖素样肽-1受体/GLP-1受体抗体
Alias	Glucagon like peptide-1 receptor; GLP 1 R; GLP 1 receptor; GLP 1R; Glp1r; GLP; glp1r; Glucagon like peptide 1 receptor; RATGL1RCP; Glip;MGC138331; GLP-1-R; GLP1R_HUMAN; Glucagon-like peptide 1 receptor; GLP-1 receptor; GLP-1-R; GLP-1R; Precursor.
literatures	Specific References  (16)     |     SL1559R has been referenced in 16 publications. [IF=5.71] Zhou, Hao, et al. "Exendin-4 protects ADMSCs from apoptosis induced by Hydrogen peroxide through PI3K/Akt-Sfrp2 pathways." Free Radical Biology and Medicine (2014).  Rat.   PubMed:25452142 [IF=5.7] Shan Y et al. The glucagon-like peptide-1 receptor agonist reduces inflammation and blood-brain barrier breakdown in an astrocyte-dependent manner in experimental stroke. J Neuroinflammation. 2019 Nov 28;16(1):242.  IHF ;  Rat.   PubMed:31779652 [IF=5.682] Haoqiang Zhang. et al. Glucagon-like peptide-1 attenuated carboxymethyl lysine induced neuronal apoptosis via peroxisome proliferation activated receptor-γ. Aging-Us. 2021 Jul 31; 13(14): 19013–19027  WB ;  Rat.   PubMed:34326274 [IF=5.64] Yuping Chen. et al. The Herb Pair Radix Rehmanniae and Cornus Officinalis Attenuated Testicular Damage in Mice With Diabetes Mellitus Through Butyric Acid/Glucagon-Like Peptide-1/Glucagon-Like Peptide-1 Receptor Pathway Mediated by Gut Microbiota. Front Microbiol. 2022 Feb 22;13:831881  IF,WB ;  Mouse.   PubMed:35273587 [IF=5.037] Zhan-zhao Liu. et al. Glucagon-like peptide-1 receptor activation by liraglutide promotes breast cancer through NOX4/ROS/VEGF pathway. Life Sci. 2022 Apr;294:120370  WB ;  Human.   PubMed:35124000 [IF=4.013] Hongzeng Li. et al. Eugenol alleviated nonalcoholic fatty liver disease in rat via a gut-brain-liver axis involving glucagon-like Peptide-1. ARCH BIOCHEM BIOPHYS. 2022 May;:109269  WB,IF ;  Rat.   PubMed:35508252 [IF=3.841] Qiaoji Tian. et al. Glucagon-Like Peptide-1 Receptor Mediates the Improvement in Glycolipid Metabolism Disorder via AKT and AMPK Signalling Pathways in L02 Cells with Insulin Resistance. J MOL STRUCT. 2022 Oct;:134328  IHC ;  Human.   PubMed:10.1016/j.molstruc.2022.134328 [IF=3.647] Melek T et al. Ileal interposition improves metabolic syndrome parameters in a rat model of metabolic syndrome induced by monosodium glutamateLife Sci.2020 Dec 9;266:118846.  WB ;  Rat.   PubMed:33309719 [IF=3.469] Shuangli Yang. et al. Glucagon-like peptide-1 alleviates diabetic kidney disease through activation of autophagy by regulating AMP-activated protein kinase-mammalian target of rapamycin pathway. Am J Physiol-Endoc M. 2020 Nov;319(6):E1019-E1030  WB,IF ;  Rat.   PubMed:32985256 [IF=3.288] Kanda et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. (2018) BMC.Cancer. 18:657  WB ;  Human.   PubMed:29907137 [IF=3.124] Wu Li. et al. GLP1R inhibits the progression of endometrial carcinoma through activation of cAMP/PKA pathway. J CLIN LAB ANAL. 2022 Aug;:e24604  WB ;  Human.   PubMed:35989517 [IF=3.06] Nagai, Takuo, et al. "Linagliptin Ameliorates Methylglyoxal-Induced Peritoneal Fibrosis in Mice." PLOS ONE 11.8 (2016): e0160993.  WB, IHC-P ;  Mouse.   PubMed:27513960 [IF=2.559] Wen Y et al. Inhibitory effects of glucagon-like peptide-1 receptor on epilepsy. Biochem Biophys Res Commun. 2019 Mar 26;511(1):79-86.  IP,WB&IHC ;  Rat&Human.   PubMed:30770099 [IF=2.395] Kim SJ et al. DPP-4 inhibition enhanced renal tubular and myocardial GLP-1 receptor expression decreased in CKD with myocardial infarction.BMC Nephrol. 2019 Mar 1;20(1):75.  WB ;  Rat.   PubMed:30823876 [IF=2.276] Liu Jinlei. et al. Emodin Attenuated the Kidney Damage of High-Fat-Diet Mice via the Upregulation of Glucagon-Like Peptide-1 Receptor. Biomed Res Int. 2021;2021:6662704  WB ;  Mouse.   PubMed:34159197 [IF=1.448] Bułdak Ł et al. The impact of exenatide (a GLP-1 agonist) on markers of inflammation and oxidative stress in normal human astrocytes subjected to various glycemic conditions. Exp Ther Med. 2019 Apr; 17(4): 2861–2869.  WB ;  Human.   PubMed:30906473
Research Area	immunology  Growth factors and hormones  Diabetes
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	51kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from rat GLP-1R: 101-200/463 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GLP1R is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.It has been suggested that this protein influences the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. GLP1R is believed to be expressed in human pancreas,lung, brain, stomach, kidney and heart. ESTs have been isolated from skin and kidney libraries. Function: This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Subunit: May form homodimers and heterodimers with GIPR. Subcellular Location: Cell membrane; Multi-pass membrane protein. Post-translational modifications: N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER. Similarity: Belongs to the G-protein coupled receptor 2 family. SWISS: P32301 Gene ID: 25051 Database links: Entrez Gene: 2740 Human Entrez Gene: 14652 Mouse Entrez Gene: 25051 Rat Omim: 138032 Human SwissProt: P43220 Human SwissProt: O35659 Mouse SwissProt: P32301 Rat Unigene: 389103 Human Unigene: 390969 Mouse Unigene: 11408 Rat GLP-1R是GLP-1在体内作用的主要靶点,它是一个7次跨膜的G protein-coupled receptor,以cAMP为主要第二信使。GLP-1与GLP-1R结合过程是一个两结构域相互作用的过程,都与胰岛素分泌和糖代谢调节密切相关。
Product Picture	Sample: Cerebrum(Rat) Lysate at 40 ug Primary: Anti-GLP-1R (SL1559R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Sample: Liver（Mouse） lysate at 30 ug; Primary: Anti- GLP-1R (SL1559R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size: 51 kD Observed band size: 51 kD Sample: Lane 1: Pancreas (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Primary: Anti-GLP-1R (SL1559R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(SL1559R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(SL1559R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-GLP-1R antibody (SL1559R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-GLP-1R antibody (SL1559R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 3

  3Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• G

  GDelete

• 
• G

  GDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout